Yamaguchi T, Yoshikawa S, Sekine Y, Hashimoto M
J Chromatogr. 1979 May 11;173(1):147-54. doi: 10.1016/s0021-9673(01)80454-4.
A gas chromatographic method has been developed that permits the accurate and specific determination of the antianginal agent ANP-4364 in plasma. ANP-4364 is extracted with n-hexane containing ethyl chloroformate and, after a clean-up procedure, derivatized to the trichloroethyl carbamate, which is assayed on a gas chromatograph equipped with an electron-capture detector. Accurate determinations are possible over a concentration range from 1 to 50 ng/ml of ANP-4364 in plasma with a relative standard deviation of 7.5%. The minimum detectable concentration is 0.5 ng/ml. Plasma levels of ANP-4364 in dogs receiving oral (10 mg/kg) or intravenous (0.1 mg/kg) dosing have been determined.
已开发出一种气相色谱法，可准确、特异性地测定血浆中的抗心绞痛药物ANP - 4364。用含氯甲酸乙酯的正己烷萃取ANP - 4364，经过净化程序后，衍生化为氨基甲酸三氯乙酯，在配备电子捕获检测器的气相色谱仪上进行测定。在血浆中ANP - 4364浓度范围为1至50 ng/ml时，可进行准确测定，相对标准偏差为7.5%。最低检测浓度为0.5 ng/ml。已测定了接受口服（10 mg/kg）或静脉注射（0.1 mg/kg）给药的犬体内ANP - 4364的血浆水平。
