Yamaguchi T, Miyamoto M, Utsui Y, Sekine Y, Hashimoto M
J Chromatogr. 1978 Oct 11;160(1):181-90. doi: 10.1016/s0021-9673(00)91794-1.
A gas chromatographic method has been developed which enables accurate determination of a new antihypertensive agent, DU-717, in plasma. DU-717 is first extracted with ethyl acetate and, after a clean-up procedure, derivatized with peracetic acid followed by diazomethane to form 2-methyl DU-717 N-oxide (direct methylation leads to mixtures). The N-oxide is then pyrolyzed to 2-methyl DU-717 on a gas chromatograph equipped with electron-capture detection. Accurate determinations are possible over a concentration range from 10 to 150 ng/ml of DU-717 in plasma at a relative standard deviation of 6.2%. The minimum detectable concentration is 1 ng/ml. Plasma levels of DU-717 in spontaneously hypertensive and normotensive rats following single oral administrations (10 mg/kg) have also been determined.
已开发出一种气相色谱法，可准确测定血浆中的新型抗高血压药物DU-717。首先用乙酸乙酯萃取DU-717，经过净化程序后，先用过氧乙酸进行衍生化，再用重氮甲烷处理，形成2-甲基DU-717 N-氧化物（直接甲基化会产生混合物）。然后在配备电子捕获检测器的气相色谱仪上将该N-氧化物热解为2-甲基DU-717。在血浆中DU-717浓度范围为10至150 ng/ml时，可进行准确测定，相对标准偏差为6.2%。最低检测浓度为1 ng/ml。还测定了自发性高血压大鼠和正常血压大鼠单次口服给药（10 mg/kg）后血浆中DU-717的水平。
