Hurst H E, Jones D R, Jarboe C H, deBree H
Clin Chem. 1981 Jul;27(7):1210-2.
The antidepressant drug clovoxamine can be specifically and sensitively quantitated in human plasma by electron capture gas chromatography. Clovoxamine and the internal standard fluvoxamine are extracted into ethyl acetate from plasma at pH 12, back-extracted into phosphoric acid, and hydrolyzed at 90 degrees C to form ketone derivatives, which are then re-extracted into hexane for injection into the chromatograph. As little as 1 microgram of clovoxamine per liter of plasma can be measured. The coefficients of variation (CV) for th analysis at concentrations of 10 and 100 micrograms/L are respectively: within-run, 5.4% and 2.7%; between-run, 17.5% and 7.0%. When this assay was applied to plasma from individuals involved in an early clinical trial of clovoxamine, steady-state plasma concentrations ranged from 50 to 77 micrograms/L 3 h after a 50-mg oral dose of clovoxamine fumarate.
抗抑郁药氯伏胺可通过电子捕获气相色谱法在人血浆中进行特异性和灵敏的定量分析。氯伏胺和内标氟伏沙明在pH 12条件下从血浆中萃取到乙酸乙酯中，再反萃取到磷酸中，并在90℃水解形成酮衍生物，然后再萃取到己烷中注入色谱仪。每升血浆中低至1微克的氯伏胺都能被检测到。在浓度为10和100微克/升时分析的变异系数（CV）分别为：批内，5.4%和2.7%；批间，17.5%和7.0%。当该测定法应用于参与氯伏胺早期临床试验个体的血浆时，口服50毫克富马酸氯伏胺3小时后，稳态血浆浓度范围为50至77微克/升。
