Scott R P, Kucera P
J Chromatogr. 1979 Dec 20;185:27-41. doi: 10.1016/s0021-9673(00)85595-8.
A gradient system for use with microbore columns is described and the repeatability of the system determined. A simple sampling device for use with aqueous biological samples is described, and the advantages of the high mass sensitivity of microbore columns for the analysis of such samples are discussed. The sensitivity of the system is demonstrated by the determination of acetophenone in water at a level of one part per billion. The use of the apparatus for the separation of low-molecular-weight, non-ionic compounds contained in blood is also demonstrated. It is shown that excellent separations of such components can be obtained from as little as 400 microliter of serum.
描述了一种用于微径柱的梯度系统，并测定了该系统的重复性。描述了一种用于水性生物样品的简单采样装置，并讨论了微径柱对这类样品分析具有高质量灵敏度的优点。通过测定水中含量为十亿分之一水平的苯乙酮，证明了该系统的灵敏度。还展示了该仪器用于分离血液中低分子量非离子化合物的用途。结果表明，从仅400微升血清中就能出色地分离出此类成分。
