Senchenko V N, Kolbanovskaia E Iu, Bocharov A L
Mol Biol (Mosk). 1979 Nov-Dec;13(6):1377-83.
A purification method for isolating homogeneous single-strand specific nuclease S1 from alpha-amyloryzin has been developed. The yield was about 16% and purification factor--9000. Nuclease S1 thus obtained was proved to be free of contaminations of any others nucleolytic enzymes. It is shown for the first time that ribo- and deoxy-dinucleosidemonophosphates are hydrolyzed by nuclease S1 to form 5'-nucleotides with pH optimum for ApA equal to 4.6.
已开发出一种从α-淀粉酶中分离纯化均一的单链特异性核酸酶S1的方法。产率约为16%，纯化因子为9000。如此获得的核酸酶S1被证明未受任何其他核酸酶的污染。首次表明，核酸酶S1可将核糖和脱氧二磷酸核苷水解形成5'-核苷酸，ApA的最适pH值为4.6。
