Thomas P T, Hinsdill R D
Drug Chem Toxicol. 1979;2(1-2):77-98. doi: 10.3109/01480547908993183.
The immunocompetence of 5 week old offspring from mice fed control chow or chow containing 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) was evaluated. The 5 ppb maternal feeding level was the only level that produced symptoms of intoxication in the offspring (i.e., facial alopecia and periorbital edema). Mice from mothers fed either 2.5 or 5 ppb of TCDD demonstrated thymus cortex atrophy and a significantly reduced spleen anti-SRBC plaque forming cell (PFC) response, but had normal serum anti-SRBC antibody levels following primary and secondary immunization. Contact sensitivity response to DNFB was significantly reduced only in offspring from mothers fed 5 ppb of TCDD. The blastogenic response of splenic T- and B-lymphocytes to concanavalin-A and E. coli lipopolysaccharide was unaffected by perinatal TCDD exposure. This correlated with the normal appearance of the T- and B-cell dependent areas of the spleens from these animals. There was no significant difference in the differential white blood cell counts between control and TCDD-exposed offspring. Offspring from mothers fed up to 5 ppb of TCDD withstood a live Listeria challenge as well as controls. However, maternal feeding levels as low as 1 ppb of TCDD rendered offspring more sensitive to an endotoxin challenge.
对喂食对照饲料或含2,3,7,8-四氯二苯并对二恶英（TCDD）饲料的小鼠5周龄后代的免疫能力进行了评估。母体喂食水平为5 ppb是唯一在后代中产生中毒症状的水平（即面部脱毛和眶周水肿）。喂食2.5或5 ppb TCDD的母鼠所产小鼠表现出胸腺皮质萎缩和脾脏抗绵羊红细胞空斑形成细胞（PFC）反应显著降低，但在初次和二次免疫后血清抗绵羊红细胞抗体水平正常。仅在喂食5 ppb TCDD的母鼠所产后代中，对二硝基氟苯（DNFB）的接触敏感性反应显著降低。围产期TCDD暴露未影响脾T淋巴细胞和B淋巴细胞对刀豆球蛋白A和大肠杆菌脂多糖的增殖反应。这与这些动物脾脏中T细胞和B细胞依赖区的正常外观相关。对照后代和TCDD暴露后代的白细胞分类计数无显著差异。喂食高达5 ppb TCDD的母鼠所产后代与对照一样能抵抗活李斯特菌的攻击。然而，母体喂食低至1 ppb的TCDD会使后代对内毒素攻击更敏感。
