Hinsdill R D, Couch D L, Speirs R S
J Environ Pathol Toxicol. 1980 Sep;4(2-3):401-25.
Juvenile and adult mice (4 and 7 weeks old, respectively) were fed various levels of 2, 3, 7, 8-tetracholorodibenzo-p-dioxin (TCDD) incorporated in mouse feed for five weeks or more. Animal parameters monitored included body weight, organ weights, white blood counts, hematocrits, certain serum protein levels (see below), symptoms of overt toxicity and mortality. High exposure levels (100 ppb) produced marked suppression in total serum protein, gamma globulin and albumin, but an increase in the beta-globulins. Feeding levels of 10 ppb TCDD or more reduced the primary and secondary antibody response to both tetanus toxoid and sheep erythrocytes. The amount of suppression appeared to be dose related, with juvenile animals showing greater suppression than adults. Antibody suppression from the 10 ppb feed level was roughly equivalent to that observed from a single high dose (200 mg/kg) of cyclophosphamide (CY). No evidence of enhanced IgE synthesis was obtained from TCDD exposed animals. TCDD feeding also lowered contact sensitization to dinitrofluorobenzene and resistance to challenge with either Salmonella typhimurium or Listeria monocytogenes.
分别给幼年和成年小鼠（分别为4周龄和7周龄）喂食添加了不同水平2,3,7,8 - 四氯二苯并 - 对 - 二恶英（TCDD）的小鼠饲料，持续五周或更长时间。监测的动物参数包括体重、器官重量、白细胞计数、血细胞比容、某些血清蛋白水平（见下文）、明显毒性症状和死亡率。高暴露水平（100 ppb）导致血清总蛋白、γ球蛋白和白蛋白显著降低，但β球蛋白增加。喂食10 ppb或更高水平的TCDD会降低对破伤风类毒素和绵羊红细胞的初次和二次抗体反应。抑制程度似乎与剂量相关，幼年动物比成年动物表现出更大的抑制。来自10 ppb饲料水平的抗体抑制作用大致等同于从单次高剂量（200 mg/kg）环磷酰胺（CY）观察到的抑制作用。未从暴露于TCDD的动物中获得IgE合成增强的证据。喂食TCDD还降低了对二硝基氟苯的接触敏感性以及对鼠伤寒沙门氏菌或单核细胞增生李斯特菌攻击的抵抗力。
