Rommel F A, Stolfi R
Immunology. 1968 Oct;15(4):469-79.
A reagent has been prepared which contains C′, C′, C′, C′, C′ and C′ by chromatography of guinea-pig serum on CM-cellulose. With this reagent, when supplemented with additional C′, EAC′ was prepared from EAC′. The reactivity and stability of cells possessing active C′ sites prepared with this reagent appeared to be the same as EAC′ prepared from EAC′ and mixtures of individual functionally pure components. The reactivity of cells possessing active C′ sites has been measured by initial velocity of generation of C′ sites, and an inhibitor of this reaction, Antrypol, has been described. A method for the estimation of the average number of C′ sites was investigated and a correlation appears to exist between the average number of sites per cell and the initial velocity of reaction with C′. Finally, a provisional method for the assay of C′ and C′ has been described.
通过用豚鼠血清在CM - 纤维素上进行色谱分析，制备了一种含有C′、C′、C′、C′、C′和C′的试剂。使用该试剂，在补充额外的C′时，由EAC′制备出了EAC′。用该试剂制备的具有活性C′位点的细胞的反应性和稳定性，似乎与由EAC′以及各个功能纯组分的混合物制备的EAC′相同。具有活性C′位点的细胞的反应性通过C′位点产生的初始速度来测量，并且已经描述了该反应的一种抑制剂——抗胰蛋白酶。研究了一种估计C′位点平均数量的方法，并且每个细胞的位点平均数量与C′反应的初始速度之间似乎存在相关性。最后，描述了一种测定C′和C′的暂行方法。
