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[血凝抑制反应在母鸡新城疫诊断中的价值]

[Diagnostic value of the hemagglutination inhibition reaction in Newcastle disease of hens].

作者信息

Pachev S, Kolarov S

出版信息

Vet Med Nauki. 1979;16(10):8-13.

PMID:549267
Abstract

Investigations were carried out using RPHA on 5299 blood serums, originating from 235 hen flocks vaccinated in four ways: Flox and orally with the vaccine strain La Sotta, intramusculary with Komarov's vaccine strain and by aerosol using the strains La Sotta and Komarov. Two hundred and eleven blood serums originating from 12 flocks, in which fowl pest was detected, were also investigated. Postinfection titer dynamics was followed in three flocks containing 20000 birds. It was established that aerosol vaccination results in highest mean geometrical titers--log2 6.3 on the average; intramuscular vaccination--in log2 5.9, oral vaccination--in log2 5.3, and Flox vaccination--in log2 4.7 on the average. The maximal value of the vaccine titers did not surpass log210, while post infection titers were of higher order and reached up to log214, which is of diagnostic value. Postinfection titers of higher than log210 value were preserved up to 5 months from the beginning of the disease, a fact of retrospective diagnostic value.

摘要

使用反向被动血凝试验(RPHA)对5299份血清样本进行了检测,这些血清来自235个鸡群,这些鸡群通过四种方式进行了疫苗接种:采用Flox方法并口服疫苗株La Sotta、肌肉注射科马罗夫疫苗株以及通过气溶胶使用La Sotta株和科马罗夫株。还对来自12个已检测出禽瘟的鸡群的211份血清样本进行了检测。对三个包含20000只鸡的鸡群追踪了感染后的滴度动态变化。结果表明,气溶胶疫苗接种导致的平均几何滴度最高——平均为log2 6.3;肌肉注射疫苗接种——平均为log2 5.9,口服疫苗接种——平均为log2 5.3,而Flox疫苗接种——平均为log2 4.7。疫苗滴度的最大值未超过log210,而感染后的滴度更高,可达log214,这具有诊断价值。感染后的滴度高于log210的值从疾病开始起可维持5个月,这一事实具有回顾性诊断价值。

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