大脑中的三羧酸循环代谢。氟乙酸和氟柠檬酸对谷氨酸、天冬氨酸、谷氨酰胺和γ-氨基丁酸标记的影响。
Tricarboxylic acid-cycle metabolism in brain. Effect of fluoroacetate and fluorocitrate on the labelling of glutamate, aspartate, glutamine and gamma-aminobutyrate.
作者信息
Clarke D D, Nicklas W J, Berl S
出版信息
Biochem J. 1970 Nov;120(2):345-51. doi: 10.1042/bj1200345.
Abstract
- The effect of fluoroacetate and fluorocitrate on the compartmentation of the glutamate-glutamine system was studied in brain slices with l-[U-(14)C]glutamate, l-[U-(14)C]aspartate, [1-(14)C]acetate and gamma-amino[1-(14)C]butyrate as precursors and in homogenates of brain tissue with [1-(14)C]acetate. The effect of fluoroacetate was also studied in vivo in mouse brain with [1-(14)C]acetate as precursor. 2. Fluoroacetate and fluorocitrate inhibit the labelling of glutamine from all precursors but affect the labelling of glutamate to a much lesser extent. This effect is not due to inhibition of glutamine synthetase. It is interpreted as being due to selective inhibition of the metabolism of a small pool of glutamate that preferentially labels glutamine.
摘要
- 以L-[U-(14)C]谷氨酸、L-[U-(14)C]天冬氨酸、[1-(14)C]乙酸盐和γ-氨基[1-(14)C]丁酸盐作为前体，在脑片中研究氟乙酸盐和氟柠檬酸盐对谷氨酸-谷氨酰胺系统区室化的影响，并以[1-(14)C]乙酸盐在脑组织匀浆中进行研究。还以[1-(14)C]乙酸盐作为前体，在小鼠脑内体内研究氟乙酸盐的作用。2. 氟乙酸盐和氟柠檬酸盐抑制所有前体对谷氨酰胺的标记，但对谷氨酸标记的影响程度要小得多。这种作用并非由于谷氨酰胺合成酶的抑制。其被解释为是由于对一小部分优先标记谷氨酰胺的谷氨酸代谢的选择性抑制。
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