通过将抹香鲸肌红蛋白偶联到多种不溶性聚合物上制备的免疫吸附剂的比较。
Comparison of immunoadsorbents prepared by coupling sperm-whale myoglobin to a variety of insoluble polymers.
作者信息
Boegman R J, Crumpton M J
出版信息
Biochem J. 1970 Nov;120(2):373-9. doi: 10.1042/bj1200373.
Abstract
Immunoadsorbents that were potentially suitable for the fractionation of antibodies to myoglobin were prepared by coupling myoglobin to aminoethylcellulose, bromoacetylcellulose, CM-cellulose, an ethylene-maleic anhydride co-polymer and Sepharose 4B. A comparison of the properties of the preparations indicated that Sepharose-myoglobin was superior, as an immunoadsorbent, to the other conjugates. Elution of adsorbed antibodies with m-propionic acid, pH2.5, gave yields of precipitable antibody higher than those achieved with other dissociating solvents. Propionic acid eluted 60-100% of the antibody adsorbed by Sepharose-myoglobin, and 60-90% of the eluted protein was precipitable antibody.
摘要
通过将肌红蛋白与氨乙基纤维素、溴乙酰纤维素、羧甲基纤维素、乙烯-马来酸酐共聚物和琼脂糖4B偶联，制备了可能适用于分离抗肌红蛋白抗体的免疫吸附剂。对这些制剂特性的比较表明，作为免疫吸附剂，琼脂糖-肌红蛋白优于其他偶联物。用pH2.5的间丙酸洗脱吸附的抗体，可沉淀抗体的回收率高于用其他解离溶剂时的回收率。丙酸洗脱了琼脂糖-肌红蛋白吸附的60-100%的抗体，洗脱蛋白的60-90%是可沉淀抗体。
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