Boegman R J, Crumpton M J
Biochem J. 1970 Nov;120(2):373-9. doi: 10.1042/bj1200373.
Immunoadsorbents that were potentially suitable for the fractionation of antibodies to myoglobin were prepared by coupling myoglobin to aminoethylcellulose, bromoacetylcellulose, CM-cellulose, an ethylene-maleic anhydride co-polymer and Sepharose 4B. A comparison of the properties of the preparations indicated that Sepharose-myoglobin was superior, as an immunoadsorbent, to the other conjugates. Elution of adsorbed antibodies with m-propionic acid, pH2.5, gave yields of precipitable antibody higher than those achieved with other dissociating solvents. Propionic acid eluted 60-100% of the antibody adsorbed by Sepharose-myoglobin, and 60-90% of the eluted protein was precipitable antibody.
通过将肌红蛋白与氨乙基纤维素、溴乙酰纤维素、羧甲基纤维素、乙烯-马来酸酐共聚物和琼脂糖4B偶联，制备了可能适用于分离抗肌红蛋白抗体的免疫吸附剂。对这些制剂特性的比较表明，作为免疫吸附剂，琼脂糖-肌红蛋白优于其他偶联物。用pH2.5的间丙酸洗脱吸附的抗体，可沉淀抗体的回收率高于用其他解离溶剂时的回收率。丙酸洗脱了琼脂糖-肌红蛋白吸附的60-100%的抗体，洗脱蛋白的60-90%是可沉淀抗体。
