Anderson E
J Cell Biol. 1970 Dec;47(3):711-33. doi: 10.1083/jcb.47.3.711.
While the ooplasmic components of centrifuged eggs of Arbacia punctulata do not stratify in homogeneous layers, we have obtained the following strata beginning with the centripetal end: lipid droplets, pronucleus, clear zone, mitochondria, yolk, and pigment. Whereas mitochondria may be found mingled with yolk bodies, we have never observed lipid droplets nor pigment bodies among any of the other inclusions. The so-called clear zone contains a heterogeneous population of inclusions: annulate lamellae, heavy bodies, Golgi complexes, and rod-containing vacuoles. The peripheral cortical granules of immature (germinal vesicle stage) and of mature eggs are not dislodged from the cortical ooplasm with the centrifugal force utilized. When the eggs are treated with urethane, prior to centrifugation, the cortical granules of mature eggs abandon their peripheral position. Further centrifugation of the initially stratified eggs produces nucleated and nonnucleated halves and the centrifugation of the halves results in quarters. The cytology of the halves and quarters is discussed. The halves and quarters have been activated with either sperm or hypertonic sea water. With the exception of the nucleated halves, we were unable to obtain plutei larvae from the other fractions (red halves and quarters). We believe that the lack of development of the various fragments is a function of the balance of particular inclusions necessary for differentiation.
虽然刺海胆离心卵的卵质成分不会分层形成均匀的层次，但我们从向心端开始获得了以下层次：脂滴、原核、透明带、线粒体、卵黄和色素。线粒体可能与卵黄体混合存在，但我们从未在其他任何内含物中观察到脂滴或色素体。所谓的透明带包含多种不同的内含物：环状片层、重体、高尔基体和含杆泡。未成熟（生发泡期）和成熟卵的外周皮质颗粒不会因所用的离心力而从皮质卵质中脱离。当卵在离心前用氨基甲酸乙酯处理时，成熟卵的皮质颗粒会离开其外周位置。对最初分层的卵进一步离心会产生有核和无核的两半，对这两半进行离心会得到四分之一部分。文中讨论了两半和四分之一部分的细胞学情况。两半和四分之一部分已用精子或高渗海水激活。除了有核的两半外，我们无法从其他部分（红色的两半和四分之一部分）获得长腕幼虫。我们认为，各种片段缺乏发育是分化所需特定内含物平衡的一种表现。
