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1
Incorporation of deoxyribonucleic acid precursors by T4 deoxyribonucleic acid-protein complexes retained on glass fiber filters.T4 脱氧核糖核酸-蛋白质复合物对脱氧核糖核酸前体的掺入，该复合物保留在玻璃纤维滤膜上。
J Virol. 1970 Oct;6(4):559-62. doi: 10.1128/JVI.6.4.559-562.1970.
2
On the exonuclease activity of phage T4 deoxyribonucleic acid polymerase.关于噬菌体T4脱氧核糖核酸聚合酶的核酸外切酶活性
J Biol Chem. 1972 May 25;247(10):3139-46.
3
Deoxyribonucleic acid synthesis in bacteriophage SPO1-infected Bacillus subtilis. I. Bacteriophage deoxyribonucleic acid synthesis and fate of host deoxyribonucleic acid in normal and polymerase-deficient strains.噬菌体SPO1感染的枯草芽孢杆菌中的脱氧核糖核酸合成。I. 正常菌株和聚合酶缺陷型菌株中噬菌体脱氧核糖核酸的合成及宿主脱氧核糖核酸的命运
J Virol. 1972 Feb;9(2):263-72. doi: 10.1128/JVI.9.2.263-272.1972.
4
Biochemical analysis of the naturally repaired sections of bacteriophage T5 deoxyribonucleic acid. I. Bromodeoxyuridine incorporation into parental deoxyribonucleic acid in the absence of deoxyribonucleic acid replication.
Biochemistry. 1968 Oct;7(10):3488-98. doi: 10.1021/bi00850a025.
5
Mutants in a nonessential gene of bacteriophage T4 which are defective in the degradation of Escherichia coli deoxyribonucleic acid.噬菌体T4非必需基因中的突变体，其在降解大肠杆菌脱氧核糖核酸方面存在缺陷。
J Virol. 1971 Jan;7(1):95-105. doi: 10.1128/JVI.7.1.95-105.1971.
6
Enzymatic synthesis of deoxyribonucleic acid. XXV. Purification and properties of deoxyribonucleic acid polymerase induced by infection with phage T4.脱氧核糖核酸的酶促合成。二十五。噬菌体T4感染诱导的脱氧核糖核酸聚合酶的纯化及性质
J Biol Chem. 1968 Feb 10;243(3):627-38.
7
Separation of T-even bacteriophage deoxyribonucleic acid from host deoxyribonucleic acid by hydroxyapatite chromatography.通过羟基磷灰石色谱法从宿主脱氧核糖核酸中分离T-偶数噬菌体脱氧核糖核酸。
J Bacteriol. 1969 Apr;98(1):104-8. doi: 10.1128/jb.98.1.104-108.1969.
8
Early intracellular events in the replication of bacteriophage T4 deoxyribonucleic acid. V. Further studies on the T4 protein-deoxyribonucleic acid complex.噬菌体T4脱氧核糖核酸复制过程中的早期细胞内事件。V. 关于T4蛋白质-脱氧核糖核酸复合物的进一步研究。
J Virol. 1970 Apr;5(4):490-501. doi: 10.1128/JVI.5.4.490-501.1970.
9
Ribonucleotides covalently linked to deoxyribonucleic acid in T4 bacteriophage.核糖核苷酸与T4噬菌体中的脱氧核糖核酸共价连接。
J Virol. 1972 Nov;10(5):902-8. doi: 10.1128/JVI.10.5.902-908.1972.
10
Properties of bacteriophage T4 mutants defective in gene 30 (deoxyribonucleic acid ligase) and the rII gene.基因30（脱氧核糖核酸连接酶）和rII基因缺陷的噬菌体T4突变体的特性
J Virol. 1971 Feb;7(2):260-6. doi: 10.1128/JVI.7.2.260-266.1971.

引用本文的文献

1
Replication of T4 DNA in Escherichia coli treated with toluene.T4噬菌体DNA在经甲苯处理的大肠杆菌中的复制
J Virol. 1973 Dec;12(6):1195-203. doi: 10.1128/JVI.12.6.1195-1203.1973.

本文引用的文献

1
Dispersive transfer of the parental DNA molecule to the progeny of phage phiX-174.亲本DNA分子向噬菌体φX-174子代的分散转移。
Virology. 1959 Oct;9:260-74. doi: 10.1016/0042-6822(59)90119-9.
2
Enzymatic synthesis of deoxyribonucleic acid. IX. The polymerase formed after T2 bacteriophage infection of Escherichia coli: a new enzyme.脱氧核糖核酸的酶促合成。IX. T2噬菌体感染大肠杆菌后形成的聚合酶：一种新酶。
J Biol Chem. 1962 Feb;237:519-25.
3
A membrane-filter technique for the detection of complementary DNA.一种用于检测互补DNA的膜过滤技术。
Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6. doi: 10.1016/0006-291x(66)90447-5.
4
Early intracellular events in the replication of T4 phage DNA. I. Complex formation of replicative DNA.T4噬菌体DNA复制过程中的早期细胞内事件。I. 复制性DNA的复合物形成
Proc Natl Acad Sci U S A. 1965 Jul;54(1):273-8. doi: 10.1073/pnas.54.1.273.
5
The structural gene for deoxyribonucleic acid polymerase in bacteriophages T4 and T5.噬菌体T4和T5中脱氧核糖核酸聚合酶的结构基因。
Proc Natl Acad Sci U S A. 1965 Oct;54(4):1241-8. doi: 10.1073/pnas.54.4.1241.
6
Early intracellular events in the replication T4 phage DNA. II. Partially replicated DNA.T4噬菌体DNA复制过程中的早期细胞内事件。II. 部分复制的DNA
Proc Natl Acad Sci U S A. 1965 Aug;54(2):634-40. doi: 10.1073/pnas.54.2.634.
7
Early intracellular events in the replication of bacteriophage T4 deoxyribonucleic acid. VI. Newly synthesized proteins in the T4 protein-deoxyribonucleic acid complex.噬菌体T4脱氧核糖核酸复制过程中的早期细胞内事件。VI. T4蛋白质-脱氧核糖核酸复合物中新合成的蛋白质
J Virol. 1970 Apr;5(4):502-6. doi: 10.1128/JVI.5.4.502-506.1970.
8
Early intracellular events in the replication of bacteriophage T4 deoxyribonucleic acid. V. Further studies on the T4 protein-deoxyribonucleic acid complex.噬菌体T4脱氧核糖核酸复制过程中的早期细胞内事件。V. 关于T4蛋白质-脱氧核糖核酸复合物的进一步研究。
J Virol. 1970 Apr;5(4):490-501. doi: 10.1128/JVI.5.4.490-501.1970.
9
Unbiased participation of T4 phage DNA strands in replication.T4噬菌体DNA链在复制中的无偏差参与。
Biochem Biophys Res Commun. 1969 Apr 29;35(2):294-9. doi: 10.1016/0006-291x(69)90281-2.
10
Fractionation of the complementary strands of coliphage T4 DNA based on the asymmetric distribution of the poly U and poly U,G binding sites.基于聚U和聚U,G结合位点的不对称分布对大肠杆菌噬菌体T4 DNA互补链进行分级分离。
Virology. 1968 Apr;34(4):608-16. doi: 10.1016/0042-6822(68)90082-2.

T4 脱氧核糖核酸-蛋白质复合物对脱氧核糖核酸前体的掺入，该复合物保留在玻璃纤维滤膜上。

Incorporation of deoxyribonucleic acid precursors by T4 deoxyribonucleic acid-protein complexes retained on glass fiber filters.

作者信息

Miller R C, Kozinski A W

出版信息

J Virol. 1970 Oct;6(4):559-62. doi: 10.1128/JVI.6.4.559-562.1970.

DOI:10.1128/JVI.6.4.559-562.1970
PMID:5497903
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC376155/
Abstract

Bacteriophage T4 deoxyribonucleic acid (DNA)-protein complexes were retained preferentially on glass fiber filters. DNA polymerase activity in the complex was detected through the incorporation of (3)H-labeled DNA precursors. The primer-product DNA hybridized with both phage and Escherichia coli DNA. Density labeling experiments showed that about 30% of incorporated (3)H-deoxyadenosine triphosphate was found in DNA which hybridized with phage DNA; this DNA was found to be covalently attached to the primer DNA.

摘要

噬菌体T4脱氧核糖核酸（DNA）-蛋白质复合物优先保留在玻璃纤维滤膜上。通过掺入（3）H标记的DNA前体来检测复合物中的DNA聚合酶活性。引物-产物DNA与噬菌体和大肠杆菌DNA均杂交。密度标记实验表明，在与噬菌体DNA杂交的DNA中发现约30%掺入的（3）H-脱氧三磷酸腺苷；发现该DNA与引物DNA共价连接。