T4 脱氧核糖核酸-蛋白质复合物对脱氧核糖核酸前体的掺入，该复合物保留在玻璃纤维滤膜上。
Incorporation of deoxyribonucleic acid precursors by T4 deoxyribonucleic acid-protein complexes retained on glass fiber filters.
作者信息
Miller R C, Kozinski A W
出版信息
J Virol. 1970 Oct;6(4):559-62. doi: 10.1128/JVI.6.4.559-562.1970.
Abstract
Bacteriophage T4 deoxyribonucleic acid (DNA)-protein complexes were retained preferentially on glass fiber filters. DNA polymerase activity in the complex was detected through the incorporation of (3)H-labeled DNA precursors. The primer-product DNA hybridized with both phage and Escherichia coli DNA. Density labeling experiments showed that about 30% of incorporated (3)H-deoxyadenosine triphosphate was found in DNA which hybridized with phage DNA; this DNA was found to be covalently attached to the primer DNA.
摘要
噬菌体T4脱氧核糖核酸（DNA）-蛋白质复合物优先保留在玻璃纤维滤膜上。通过掺入（3）H标记的DNA前体来检测复合物中的DNA聚合酶活性。引物-产物DNA与噬菌体和大肠杆菌DNA均杂交。密度标记实验表明，在与噬菌体DNA杂交的DNA中发现约30%掺入的（3）H-脱氧三磷酸腺苷；发现该DNA与引物DNA共价连接。
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