Miller R C, Taylor D M, MacKay K, Smith H W
J Virol. 1973 Dec;12(6):1195-203. doi: 10.1128/JVI.12.6.1195-1203.1973.
Deoxyribonucleoside triphosphates are incorporated into T4 DNA in infected cells treated with toluene. Under the proper conditions the incorporation is controlled by the known T4 DNA polymerase and proceeds by a semiconservative mechanism. Both strands of the phage DNA are replicated into a high molecular weight progeny molecule. The replication system is accessible to extracellular pancreatic DNase added to the reaction mixture. At early times after infection a second replication system, not under control of the gene 43 polymerase, has been detected which synthesizes T4 DNA in toluenized cells.
脱氧核糖核苷三磷酸在经甲苯处理的受感染细胞中掺入到T4 DNA中。在适当条件下，掺入由已知的T4 DNA聚合酶控制，并通过半保留机制进行。噬菌体DNA的两条链都复制成高分子量的子代分子。添加到反应混合物中的细胞外胰脱氧核糖核酸酶可作用于该复制系统。在感染后的早期，已检测到第二个复制系统，它不受基因43聚合酶的控制，可在经甲苯处理的细胞中合成T4 DNA。
