四乙铵离子对神经中延迟钾电流的选择性抑制。
The selective inhibition of delayed potassium currents in nerve by tetraethylammonium ion.
作者信息
Hille B
出版信息
J Gen Physiol. 1967 May;50(5):1287-302. doi: 10.1085/jgp.50.5.1287.
Abstract
The effect of tetraethylammonium ion (TEA) on the voltage clamp currents of nodes of Ranvier of frog myelinated nerve fibers is studied. The delayed K currents can be totally abolished by TEA without affecting the transient Na currents or the leakage current in any way. Both inward and outward currents disappear. In low TEA concentrations small K currents remain with normal time constants. The dose-response relationship suggests the formation of a complex between TEA and a receptor with a dissociation constant of 0.4 mM. Other symmetrical quaternary ammonium ions have very little effect. There is no competition between TEA and agents that affect the Na currents such as Xylocaine, tetrodotoxin, or Ca ions. The pharmacological data demonstrate that the Na, K, and leakage permeabilities are chemically independent, probably because their mechanisms occupy different sites on the nodal membrane. The data are gathered and analyzed by digital computer.
摘要
研究了四乙铵离子（TEA）对青蛙有髓神经纤维郎飞结电压钳电流的影响。TEA可完全消除延迟性钾电流，且对瞬时钠电流或漏电流无任何影响。内向电流和外向电流均消失。在低浓度TEA时，小的钾电流仍具有正常的时间常数。剂量反应关系表明TEA与一种受体形成复合物，其解离常数为0.4 mM。其他对称季铵离子的影响很小。TEA与影响钠电流的药物如利多卡因、河豚毒素或钙离子之间不存在竞争。药理学数据表明钠、钾和漏电流的通透性在化学上是独立的，可能是因为它们的机制占据了结膜上不同的位点。数据由数字计算机收集和分析。
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