Mantei N, Boll W, Weissmann C
Nature. 1979 Sep 6;281(5726):40-6. doi: 10.1038/281040a0.
Mouse thymidine kinase-negative L cells were transformed with a cloned rabbit chromosomal beta-globin gene linked to the clone thymidine kinase gene of herpes simplex virus type 1. Most thymidine kinase-positive cell lines contained one or more copies of rabbit beta-globin DNA and produced up to 2,000 copies of rabbit beta-globin RNA per cell indistinguishable from its authentic counterpart. No mouse beta-globin mRNA was detected.
用与1型单纯疱疹病毒的克隆胸苷激酶基因相连的克隆兔染色体β-珠蛋白基因转化小鼠胸苷激酶阴性L细胞。大多数胸苷激酶阳性细胞系含有一个或多个兔β-珠蛋白DNA拷贝，每个细胞产生多达2000个兔β-珠蛋白RNA拷贝，与天然对应物无法区分。未检测到小鼠β-珠蛋白mRNA。
