Beggs J D, van den Berg J, van Ooyen A, Weissmann C
Nature. 1980 Feb 28;283(5750):835-40. doi: 10.1038/283835a0.
A 5.1 kilobase-pair segment of rabbit chromosomal beta-globin DNA was joined to pJDB219, a plasmid consisting of pMB9, the 2-mu yeast plasmid and the yeast leu-2 gene. Saccharomyces cerevisiae transformed with the globin DNA-containing hybrid produced beta-globin-specific RNA. As compared to mature beta-globin mRNA, these transcripts lacked 20-40 nucleotides from the 5' end, contained all of the small intron and extended to about the middle of the large intron. Thus, no splicing of the primary beta-globin transcript could be detected in the yeast cells.
一段5.1千碱基对的兔染色体β-珠蛋白DNA片段与pJDB219连接，pJDB219是一种由pMB9、2μm酵母质粒和酵母亮氨酸-2基因组成的质粒。用含珠蛋白DNA的杂种转化的酿酒酵母产生了β-珠蛋白特异性RNA。与成熟的β-珠蛋白mRNA相比，这些转录本在5'端缺少20 - 40个核苷酸，包含所有小内含子，并延伸至大约大内含子的中部。因此，在酵母细胞中未检测到初级β-珠蛋白转录本的剪接。
