Breathnach R, Mantei N, Chambon P
Proc Natl Acad Sci U S A. 1980 Feb;77(2):740-4. doi: 10.1073/pnas.77.2.740.
Mouse thymidine-kinase-negative L cells were transformed with a cloned chicken ovalbumin gene linked to the cloned thymidine kinase gene from herpes simplex virus type 1. Most thymidine-kinase-positive clones contained one or more copies of the ovalbumin gene, which were stably maintained for at least 6 months during continuous culture under selective conditions. Transcription of the ovalbumin gene was detected at a low level, producing RNA molecules that were correctly spliced and polyadenylylated by comparison with genuine ovalbumin mRNA. However, the 5' end of these RNA molecules does not correspond to that of ovalbumin mRNA.
用与来自1型单纯疱疹病毒的克隆胸苷激酶基因相连的克隆鸡卵清蛋白基因转化小鼠胸苷激酶阴性L细胞。大多数胸苷激酶阳性克隆含有一个或多个卵清蛋白基因拷贝，在选择性条件下连续培养期间，这些拷贝稳定维持至少6个月。在低水平检测到卵清蛋白基因的转录，产生的RNA分子与真正的卵清蛋白mRNA相比，经过了正确的剪接和聚腺苷酸化。然而，这些RNA分子的5'端与卵清蛋白mRNA的5'端不对应。
