Sun G Y, Creech D M, Sun A Y
Curr Alcohol. 1979;7:61-9.
An assay of cholesteryl ester hydrolase activity of microsomes from guinea pig adrenal indicated maximal activity at pH at pH 4.0 and 5.5. When ethanol was added to the microsomes, these enzymes showed differences in response with increasing ethanol concentration. Rats administered 15% ethanol as drink for 4--5 months showed a small (20%) increase in cholesteryl ester hydrolase (pH 4.0) activity in adrenal as compared to controls. However, a 50% decrease in cholesteryl ester hydrolase activity resulted when rats were administered ethanol by intragastric tubing technique (15 days) as compared to controls treated similarly with saline. The decrease in cholesteryl ester hydrolase activity was correlated with an increase in cholesteryl ester level in serum. In a different study, rats intubated with an acute dose of ethanol showed an increase in adrenal cholesteryl ester hydrolase activity as early as 1 hr after administration. This effect seemed to last for about 24 hr before activity returned to control level. It is concluded that different modes of ethanol administration to rats may elicit a change in cholesteryl ester hydrolase activity in the adrenal. An increase in cholesteryl ester hydrolase activity may be related to an increase in steroidogenic activity and activation of adrenocortical function.
对豚鼠肾上腺微粒体胆固醇酯水解酶活性的测定表明，在pH 4.0和5.5时活性最高。当向微粒体中加入乙醇时，这些酶随着乙醇浓度的增加表现出不同的反应。以15%乙醇为饮品喂食4至5个月的大鼠，其肾上腺中胆固醇酯水解酶（pH 4.0）的活性与对照组相比有小幅（20%）增加。然而，与用生理盐水进行类似处理的对照组相比，通过胃管技术给大鼠喂食乙醇（15天）后，胆固醇酯水解酶活性降低了50%。胆固醇酯水解酶活性的降低与血清中胆固醇酯水平的升高相关。在另一项研究中，急性给予乙醇灌胃的大鼠在给药后1小时肾上腺胆固醇酯水解酶活性就出现增加。这种效应似乎持续约24小时，之后活性恢复到对照水平。得出的结论是，对大鼠采用不同的乙醇给药方式可能会引起肾上腺中胆固醇酯水解酶活性的变化。胆固醇酯水解酶活性的增加可能与类固醇生成活性的增加和肾上腺皮质功能的激活有关。
