一种用于低温研究的新型分光光度比色皿架；其在一氧化碳血红蛋白氧化速率研究中的应用。

A new spectrophotometric cuvette holder for low temperature studies; its application to the study of carbonmonoxyhemoglobin oxidation rate.

作者信息

Mosca A, Musetti A, Samaja M

出版信息

J Biochem Biophys Methods. 1979 Dec;1(6):319-26. doi: 10.1016/0165-022x(79)90022-8.

Abstract

A new spectrophotometric cuvette holder to be used for subzero temperature is described. The device is easily adaptable to a commercial spectrophotometer and it was checked down to --40 degrees C. Satisfactory mixing of the reactants contained in the cuvette at low temperatures is attained using a special stirrer and suitable solution volumes. The rate of carbonmonoxyhemoglobin oxidation by K3Fe(CN)6 at different subzero temperatures has been studied using this apparatus; the results are in agreement with the extrapolated data at room temperature.

摘要

描述了一种用于零下温度的新型分光光度比色皿架。该装置易于适配商用分光光度计，并且已在低至零下40摄氏度的温度下进行了测试。使用特殊搅拌器和合适的溶液体积，可在低温下使比色皿中的反应物充分混合。利用该装置研究了在不同零下温度下K3Fe(CN)6氧化碳氧血红蛋白的速率；结果与室温下的外推数据一致。