Abo T, Kumagai K
Clin Exp Immunol. 1978 Sep;33(3):441-52.
The levels of B lymphocytes bearing surface immunoglobulins in human peripheral blood were measured by immunofluorescence after pre-treatment of the cells with a low pH shock or washing at 37 degrees C. It was found that the percentages of SIg-bearing cells in an individual varied throughout the day and showed a circadian rhythm with a peak at 12 midnight and a depression at 12 noon. In addition, the levels of the B lymphocytes in the peripheral blood were shown to vary annually, being lower in the winter than in the summer.
在对人外周血中的细胞进行低pH冲击预处理或在37℃下洗涤后,通过免疫荧光法测定携带表面免疫球蛋白的B淋巴细胞水平。结果发现,个体中携带表面免疫球蛋白(SIg)的细胞百分比在一天中有所变化,并呈现出昼夜节律,午夜12点达到峰值,中午12点出现低谷。此外,外周血中的B淋巴细胞水平显示出每年都有变化,冬季低于夏季。