用于研究由传染性胃肠炎冠状病毒（TGEV）诱导的表面抗原的固定细胞免疫过氧化物酶技术。
Fixed-cell immunoperoxidase technique for the study of surface antigens induced by the coronavirus of transmissible gastroenteritis (TGEV).
作者信息
To L T, Bernard S, Lantier I
机构信息
Laboratory of Virology, Institute of Veterinary Research, Bach mai-Hànôi, Viêtnam.
出版信息
Vet Microbiol. 1991 Nov;29(3-4):361-8. doi: 10.1016/0378-1135(91)90143-4.
Abstract
An immunoperoxidase technique performed on the TGEV-infected cells was developed for detection of virus-induced antigens. The presence of M antigen of TGEV on the surface of infected cells was demonstrated by this technique. This finding is in contrast to the M protein of murine hepatitis coronavirus which migrates to the Golgi apparatus but is not transported to the plasma membrane. The time course of appearance M and S antigens on the surface of TGEV-infected cell can be studied by this technique.
摘要
为检测病毒诱导的抗原，开发了一种在感染猪传染性胃肠炎病毒（TGEV）的细胞上进行的免疫过氧化物酶技术。通过该技术证明了感染细胞表面存在TGEV的M抗原。这一发现与鼠肝炎冠状病毒的M蛋白不同，后者迁移至高尔基体，但不会转运至质膜。通过该技术可以研究TGEV感染细胞表面M和S抗原出现的时间进程。
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