Fair W R, Wehner N
Appl Microbiol. 1971 Jan;21(1):6-8. doi: 10.1128/am.21.1.6-8.1971.
The basic polyamine spermine was tested for antibacterial activity at two pH levels by the modified cup method against a variety of gram-positive and gram-negative organisms isolated from urine. At pH 6.4, with concentrations ranging from 39 to 2,500 mug per 0.1 ml, there were no clear zones of inhibition seen with any of the gram-negative test organisms, although some adverse effect on growth within the area of the cylinder was noted in 36%. Three of 17 gram-positive strains were inhibited at this pH. Spermine was more active at pH 7.4, but even at the highest concentrations only 16% of the gram-negative and 47% of the gram-positive bacteria tested showed definite zones of inhibition. It is concluded that spermine probably plays little, if any, role in natural resistance to urinary tract infections in vivo.
采用改良杯碟法，在两个pH水平下检测了碱性多胺精胺对从尿液中分离出的多种革兰氏阳性菌和革兰氏阴性菌的抗菌活性。在pH 6.4时，每0.1 ml精胺浓度范围为39至2500μg，所有革兰氏阴性受试菌均未出现明显抑菌圈，不过在36%的受试菌中，观察到圆柱体区域内的生长受到了一些不利影响。在该pH值下，17株革兰氏阳性菌中有3株受到抑制。精胺在pH 7.4时活性更高，但即使在最高浓度下，受试的革兰氏阴性菌中只有16%、革兰氏阳性菌中只有47%显示出明确的抑菌圈。结论是，精胺在体内对尿路感染的天然抵抗力中可能几乎不起作用（如果有作用的话也很小）。
