Murgier M, Belaich J P
J Bacteriol. 1971 Feb;105(2):573-9. doi: 10.1128/jb.105.2.573-579.1971.
A microcalorimetric method using a Tian-Calvet apparatus was utilized for measuring the K(m) and V(max) of intact yeast cells for some carbohydrate substrates. These values were obtained for fructose, galactose, and maltose. The heats of ethanolic fermentations were also directly determined, and the results were very close to those obtained by using theoretical calculations and standard values.
采用天-卡尔维量热仪的微量热法用于测定完整酵母细胞对某些碳水化合物底物的米氏常数(Km)和最大反应速率(Vmax)。得到了果糖、半乳糖和麦芽糖的这些值。还直接测定了乙醇发酵热,结果与通过理论计算和标准值得到的结果非常接近。