大肠杆菌厌氧生长的微量量热研究：全细胞对各种能量底物亲和力的测量。
Microcalorimetric study of the anaerobic growth of Escherichia coli: measurements of the affinity of whole cells for various energy substrates.
作者信息
Belaich A, Belaich J P
出版信息
J Bacteriol. 1976 Jan;125(1):19-24. doi: 10.1128/jb.125.1.19-24.1976.
Abstract
Microcalorimetry has been used to determine the affinity of whole cells of Escherichia coli for glucose, galactose, fructose, and lactose. Anaerobic growth thermograms were analyzed, and the Km and Vmax values for these energy substrates were measured at pH 7.8. Results obtained with this technique using various organisms growing anaerobically on different sugars are compared. This comparison shows that in practically all cases the cellular rate of catabolic activity is a hyperbolic function of the energy substrate concentrations at low sugar concentrations. In some cases this technique also allows determination of kinetics at high sugar concentrations.
摘要
微量热法已被用于测定大肠杆菌全细胞对葡萄糖、半乳糖、果糖和乳糖的亲和力。分析了厌氧生长热谱图，并在pH 7.8条件下测量了这些能量底物的米氏常数（Km）和最大反应速率（Vmax）值。比较了使用该技术在不同糖类上厌氧生长的各种生物体所获得的结果。这种比较表明，在几乎所有情况下，在低糖浓度下，细胞分解代谢活性速率是能量底物浓度的双曲线函数。在某些情况下，该技术还可以测定高糖浓度下的动力学。
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