微生物细胞中葡萄糖渗透的微量热研究。
Microcalorimetric study of glucose permeation in microbial cells.
作者信息
Belaich J P, Senez J C, Murgier M
出版信息
J Bacteriol. 1968 May;95(5):1750-7. doi: 10.1128/jb.95.5.1750-1757.1968.
Abstract
A microcalorimetric method for measuring the influence of extracellular glucose concentration on the rate of catabolism is described. This method has been applied to anaerobically growing cultures of Zymomonas mobilis and of a respiratory-deficient ("petite") mutant of Saccharomyces cerevisiae (strain YFa). The Michaelian kinetics recorded with both organisms were apparently related to glucose transport. With Z. mobilis, it was found that, in the range of glucose concentrations at which this organism was growing exponentially, cell activity was limited by the maximal rate of the catabolic enzymes; at lower concentrations, glucose transport was the rate controlling step. The metabolic activity of yeast always depended on external glucose concentration; when this was lowered under a threshold, a change of kinetics took place. The microcalorimetric method described seems to be widely applicable to kinetic studies of the permeation of metabolizable substrates in microorganisms.
摘要
本文描述了一种用于测量细胞外葡萄糖浓度对分解代谢速率影响的微量量热法。该方法已应用于运动发酵单胞菌和酿酒酵母呼吸缺陷型（“小菌落”）突变体（YFa菌株）的厌氧培养物。两种生物体记录的米氏动力学显然与葡萄糖转运有关。对于运动发酵单胞菌，发现在该生物体指数生长的葡萄糖浓度范围内，细胞活性受分解代谢酶的最大速率限制；在较低浓度下，葡萄糖转运是速率控制步骤。酵母的代谢活性始终取决于外部葡萄糖浓度；当葡萄糖浓度降至阈值以下时，动力学发生变化。所描述的微量量热法似乎广泛适用于微生物中可代谢底物渗透的动力学研究。
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本文引用的文献
1
2
INFLUENCE OF AERATION AND OF PANTOTHENATE ON GROWTH YIELDS OF ZYMOMONAS MOBILIS.通气和泛酸盐对运动发酵单胞菌生长产量的影响
J Bacteriol. 1965 May;89(5):1195-200. doi: 10.1128/JB.89.5.1195-1200.1965.
3
THE GLUCOSE PERMEASE SYSTEM IN BACTERIA.细菌中的葡萄糖通透酶系统
Biochim Biophys Acta. 1964 Mar 30;79:337-50.
4
UPTAKE OF AMINO ACIDS BY SALMONELLA TYPHIMURIUM.鼠伤寒沙门氏菌对氨基酸的摄取
Arch Biochem Biophys. 1964 Jan;104:1-18. doi: 10.1016/s0003-9861(64)80028-x.
5
Mechanism of glucose transport across the yeast cell membrane.葡萄糖跨酵母细胞膜的转运机制。
J Bacteriol. 1962 Sep;84(3):485-91. doi: 10.1128/jb.84.3.485-491.1962.
6
[Glucose-limiting thermogenesis and growth of Pseudomonas lindneri].[葡萄糖限制下林氏假单胞菌的产热与生长]
C R Seances Soc Biol Fil. 1963 Jun 10;157:316-22.
7
Enthalpy changes associated with the lactic fermentation of glucose.与葡萄糖乳酸发酵相关的焓变。
J Bacteriol. 1961 Nov;82(5):685-90. doi: 10.1128/jb.82.5.685-690.1961.
8
The characterization of the pathway of maltose utilization by Escherichia coli. III. Adescription of the concentrating mechanism.大肠杆菌利用麦芽糖途径的特性。III. 浓缩机制的描述。
Biochim Biophys Acta. 1960 Apr 22;39:440-7. doi: 10.1016/0006-3002(60)90196-7.
9
The concept of carrier transport and its corollaries in pharmacology.载体转运的概念及其在药理学中的必然结果。
Pharmacol Rev. 1961 Jun;13:109-83.
10
The growth of micro-organisms in relation to their energy supply.微生物生长与其能量供应的关系。
J Gen Microbiol. 1960 Dec;23:457-69. doi: 10.1099/00221287-23-3-457.
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