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大肠杆菌有氧生长的微量量热研究:动力学及与琥珀酸生长相关的实验焓

Microcalorimetric study of Escherichia coli aerobic growth: kinetics and experimental enthalpy associated with growth on succinic acid.

作者信息

Dermoun Z, Belaich J P

出版信息

J Bacteriol. 1979 Nov;140(2):377-80. doi: 10.1128/jb.140.2.377-380.1979.

Abstract

A new batch calorimeter was used to study the aerobic growth of Escherichia coli in a minimal containing a growth-limiting concentration of succinic acid. The shape of the thermograms obtained was simple when the energy source was the only growth-limiting factor and showed a stationary phase when the oxygen transfer was limiting. The experimentally determined value of the heat production during growth was found to be significantly lower than the heat of combustion of the succinic acid corrected for the assimilated-substrate fraction. An interpretation has been given to explain the difference between the experimental and the theoretical values.

摘要

使用一种新型批量量热计来研究大肠杆菌在含有生长限制浓度琥珀酸的基本培养基中的需氧生长。当能量源是唯一的生长限制因素时,所获得的热谱图形状简单,而当氧气传递受到限制时则呈现出稳定期。发现生长过程中实验测定的产热值明显低于根据同化底物分数校正后的琥珀酸燃烧热。已给出一种解释来说明实验值与理论值之间的差异。

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