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大肠杆菌有氧生长的微量量热研究:动力学及与琥珀酸生长相关的实验焓

Microcalorimetric study of Escherichia coli aerobic growth: kinetics and experimental enthalpy associated with growth on succinic acid.

作者信息

Dermoun Z, Belaich J P

出版信息

J Bacteriol. 1979 Nov;140(2):377-80. doi: 10.1128/jb.140.2.377-380.1979.

DOI:10.1128/jb.140.2.377-380.1979
PMID:387732
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC216659/
Abstract

A new batch calorimeter was used to study the aerobic growth of Escherichia coli in a minimal containing a growth-limiting concentration of succinic acid. The shape of the thermograms obtained was simple when the energy source was the only growth-limiting factor and showed a stationary phase when the oxygen transfer was limiting. The experimentally determined value of the heat production during growth was found to be significantly lower than the heat of combustion of the succinic acid corrected for the assimilated-substrate fraction. An interpretation has been given to explain the difference between the experimental and the theoretical values.

摘要

使用一种新型批量量热计来研究大肠杆菌在含有生长限制浓度琥珀酸的基本培养基中的需氧生长。当能量源是唯一的生长限制因素时,所获得的热谱图形状简单,而当氧气传递受到限制时则呈现出稳定期。发现生长过程中实验测定的产热值明显低于根据同化底物分数校正后的琥珀酸燃烧热。已给出一种解释来说明实验值与理论值之间的差异。

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本文引用的文献

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J Bacteriol. 1961 Nov;82(5):648-56. doi: 10.1128/jb.82.5.648-656.1961.
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Microcalorimetric study of glucose permeation in microbial cells.微生物细胞中葡萄糖渗透的微量热研究。
J Bacteriol. 1968 May;95(5):1750-7. doi: 10.1128/jb.95.5.1750-1757.1968.
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Microcalorimetric determination of the affinity of Saccharomyces cerevisiae for some carbohydrate growth substrates.微量量热法测定酿酒酵母对某些碳水化合物生长底物的亲和力。
J Bacteriol. 1971 Feb;105(2):573-9. doi: 10.1128/jb.105.2.573-579.1971.
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A system of micro-calorimeters and its use in biochemistry and biology.
Biochem Soc Trans. 1976;4(4):561-5. doi: 10.1042/bst0040561a.
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Aerobic growth thermograms of Streptococcus lactis obtained with a complex medium containing glucose.用含有葡萄糖的复合培养基获得的乳酸链球菌需氧生长温度记录图。
J Bacteriol. 1978 Aug;135(2):373-8. doi: 10.1128/jb.135.2.373-378.1978.
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A microcalorimetric study of the growth of Klebsiella aerogenes in simple salts/glucose media.产气克雷伯菌在简单盐类/葡萄糖培养基中生长的微量热研究。
Microbios. 1976;16(63):37-48.
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