Spooner B S, Hilfer S R
J Cell Biol. 1971 Feb;48(2):225-34. doi: 10.1083/jcb.48.2.225.
Previous results with thyroid secretory cells in monolayer culture seem contradictory with respect to phenotypic stability of this cell type. On the one hand, in "minimal" medium the cells lose structural and functional specializations which can be returned only by three-dimensional growth in organ culture upon addition of fibroblasts derived from the thyroid capsule. On the other hand, in "rich" medium used for cloning, cytoarchitecture and function remain unaltered in either mass or clonal cultures. The apparent discrepancy has been resolved by plating cell suspensions in both media and changing to the alternate medium once the cells have become established. It has been shown that a number of characteristics, including hormone levels, are reversed each time such a change in medium is made. These modulations are discussed in terms of the normal variations in structure and function of the gland in vivo.
先前关于单层培养的甲状腺分泌细胞的研究结果，在这种细胞类型的表型稳定性方面似乎相互矛盾。一方面，在“基础”培养基中，细胞会失去结构和功能特化，只有在添加来自甲状腺被膜的成纤维细胞后，通过器官培养中的三维生长才能恢复。另一方面，在用于克隆的“丰富”培养基中，无论是大规模培养还是克隆培养，细胞结构和功能都保持不变。通过在两种培养基中接种细胞悬液，并在细胞贴壁后更换为另一种培养基，这一明显的差异得到了解决。研究表明，每次更换培养基时，包括激素水平在内的许多特性都会发生逆转。这些调节作用将根据体内甲状腺结构和功能的正常变化进行讨论。
