Gunn R B, Tosteson D C
J Gen Physiol. 1971 May;57(5):593-609. doi: 10.1085/jgp.57.5.593.
2,4,6-Trinitro-3-methyl-phenol (trinitrocresol, H(+)TNC(-)) was found to inhibit anion and stimulate cation movements across the membranes of both high potassium (HK) and low potassium (LK) sheep red blood cells. The concentration of TNC(-) required to inhibit SO(4) (-) and Cl(-) efflux (10(-5)-10(-3)M) was less than that required to increase Na(+) and K(+) leakage (10(-3)-10(-2)M). Both the inhibition of anion and stimulation of cation permeation were reversed if TNC(-) was washed from the red cells. The cation leak caused by TNC(-) was much greater at 0 degrees and 37 degrees C than at room temperature (23 degrees C). In sheep red cells, TNC(-) was found to be about 20 times more effective than salicylate and about 40 times more effective than thiocyanate in increasing cation leak. TNC(-) also inhibited the ouabain-sensitive potassium influx.
发现2,4,6-三硝基-3-甲基苯酚（三硝基甲酚，H(+)TNC(-)）可抑制阴离子并刺激高钾（HK）和低钾（LK）绵羊红细胞膜上的阳离子转运。抑制SO(4) (-)和Cl(-)外流所需的TNC(-)浓度（10(-5)-10(-3)M）低于增加Na(+)和K(+)泄漏所需的浓度（10(-3)-10(-2)M）。如果将TNC(-)从红细胞中洗去，阴离子的抑制和阳离子渗透的刺激都会逆转。TNC(-)引起的阳离子泄漏在0摄氏度和37摄氏度时比室温（23摄氏度）时大得多。在绵羊红细胞中，发现TNC(-)在增加阳离子泄漏方面比水杨酸盐有效约20倍，比硫氰酸盐有效约40倍。TNC(-)还抑制哇巴因敏感的钾内流。
