Marrack J R, Richards C B
Immunology. 1971 Jun;20(6):1019-40.
Formation of aggregates in mixtures of antigen and antibody was followed by measurement of the intensity of light scattered at 90° and of the ratio of the intensities of light scattered at 45° and 135° under varied environmental conditions—concentration, antigen/antibody ratio, temperature, concentration of neutral salts, hydrogen ion and organic solutes. We propose that the aggregation of antigen—antibody complexes is accelerated by change in the structure of antibody molecules that occurs on combination with antigen. The effect of solutes on the rate of aggregation may be due to reduction of the association constant of the combination of antigen and antibody or to inhibition of the change in structure of antibody on combination. Acceleration of the early stage of aggregation by increase of temperature is attributed to increased rate of change in the structure of antibody.
通过测量在不同环境条件下（浓度、抗原/抗体比例、温度、中性盐浓度、氢离子和有机溶质）90°散射光的强度以及45°和135°散射光强度的比值，来跟踪抗原和抗体混合物中聚集体的形成。我们提出，抗原-抗体复合物的聚集是由抗体分子与抗原结合时发生的结构变化所加速的。溶质对聚集速率的影响可能是由于抗原和抗体结合的缔合常数降低，或者是由于抑制了抗体与抗原结合时的结构变化。温度升高加速聚集早期阶段的原因是抗体结构变化的速率增加。
