Nelson D S, Nelson M, Thurston J M, Waters M F, Pearson J M
Clin Exp Immunol. 1971 Jul;9(1):33-43.
Blood lymphocytes from Malay, Indian and Chinese patients with leprosy, and from race-matched controls, were cultured in the presence of phytohaemagglutinin. Cells from Malays and Indians with lepromatous leprosy and from Malays with tuberculoid leprosy transformed as well as cells from normal controls when cultured in normal (reference) serum. Cells from lepromatous Malays and Indians transformed significantly less well than cells from normal controls when cultured in autologous serum. Normal lymphocytes transformed significantly less well when cultured in serum from lepromatous or tuberculoid Malays or from lepromatous Indians than when cultured in serum from normal controls. Lymphocytes from lepromatous Chinese transformed significantly more extensively than those from normal Chinese, whether cultured in normal (reference) or autologous serum. The ratio of transformation in autologous serum to transformation in reference serum was significantly depressed for lepromatous Chinese. Although lepromatous Chinese serum, compared with normal Chinese serum, did not depress the response of lymphocytes from one donor, there was evidence of depression when cells from another donor were used. Cells and sera from Chinese patients biopsied for suspected nasopharyngeal carcinoma behaved similarly to those from lepromatous Chinese, and not to those of normal Chinese, whether or not nasopharyngeal carcinoma was found. Lymphocytes from patients with disease classified as stable, regardless of other criteria, transformed significantly less well in either normal or autologous serum than did cells from patients with downgrading (rapidly progressive) disease. This was true of all races. In the case of Malays and Indians with stable disease the cells also transformed less well than normal cells in reference serum. The difficulties in design, execution and interpretation of studies of lymphocyte transformation in disease and the importance of humoral factors are emphasized.
将来自马来族、印度族和华裔麻风病患者以及种族匹配对照者的血液淋巴细胞在植物血凝素存在的情况下进行培养。当在正常（参考）血清中培养时，来自瘤型麻风的马来族和印度族患者以及结核样型麻风的马来族患者的细胞与正常对照者的细胞一样发生转化。当在自身血清中培养时，瘤型麻风的马来族和印度族患者的细胞转化效果明显不如正常对照者的细胞。当在瘤型或结核样型马来族患者或瘤型印度族患者的血清中培养时，正常淋巴细胞的转化效果明显不如在正常对照者血清中培养时。无论在正常（参考）血清还是自身血清中培养，瘤型华裔患者的淋巴细胞转化程度均明显高于正常华裔患者。瘤型华裔患者在自身血清中的转化与在参考血清中的转化之比明显降低。尽管与正常华裔血清相比，瘤型华裔血清不会抑制来自一名供体的淋巴细胞反应，但当使用另一名供体的细胞时，有抑制的证据。因疑似鼻咽癌而接受活检的华裔患者的细胞和血清，无论是否发现鼻咽癌，其表现都与瘤型华裔患者的相似，而与正常华裔患者的不同。无论其他标准如何，被归类为病情稳定的患者的淋巴细胞在正常血清或自身血清中的转化效果均明显不如病情恶化（快速进展）患者的细胞。所有种族都是如此。对于病情稳定的马来族和印度族患者，其细胞在参考血清中的转化效果也不如正常细胞。文中强调了疾病中淋巴细胞转化研究在设计、实施和解释方面的困难以及体液因素的重要性。
