Goorha R, Willis D B, Granoff A
J Virol. 1977 Feb;21(2):802-5. doi: 10.1128/JVI.21.2.802-805.1977.
Previous evidence indicated that frog virus 3 (FV3), an icosahedral DNA virus, replicates exclusively in the cytoplasm. However, data presented here demonstrate that FV3 does not replicate in UV-irradiated or enuleated chicken embryo or BSC-1 cells and that virus-specific DNA synthesis is not initiated in such cells. Primary transcription was not detected in infected enucleated cells. These results demonstrate that a functional nucleus is essential for FV3 replication.
先前的证据表明,蛙病毒3(FV3)是一种二十面体DNA病毒,仅在细胞质中复制。然而,此处给出的数据表明,FV3在紫外线照射或去核的鸡胚或BSC-1细胞中不复制,并且在这些细胞中不会启动病毒特异性DNA合成。在感染的去核细胞中未检测到初级转录。这些结果表明,功能性细胞核对于FV3复制至关重要。