微丝束和细胞形态与对基质的粘附性有关，并且在培养的成纤维细胞中可与生长控制分离。

Microfilament bundles and cell shape are related to adhesiveness to substratum and are dissociable from growth control in cultured fibroblasts.

作者信息

Willingham M C, Yamada K M, Yamada S S, Pouysségur J, Pastan I

出版信息

Cell. 1977 Mar;10(3):375-80. doi: 10.1016/0092-8674(77)90024-1.

Abstract

The distribution of microfilament bundles in cells was examined using antibodies to fibroblast myosin and indirect immunofluorescence microscopy. There is no correlation between the presence of bundles of microfilaments and normal growth control. A normal cell line (Balb/c 3T3) cultured on a poorly adhesive substratum showed no microfilament bundles. Similarly, a mutant cell line (AD6) with normal growth, but a rounded shape due to defective adhesiveness to substratum, showed no bundle formation. On the other hand, two transformed cell lines with a flat morphology (Swiss SV3T3 and Balb MSV-85) showed extensive bundle formation. When a transformed cell line with poor adhesiveness (MC5-5) was treated with CSP (a major surface glycoprotein of normal cells) which increases adhesiveness to substratum, the cells formed extensive microfilament bundles without any decrease in growth. We conclude that the distribution of microfilament bundles is related to adhesiveness to substratum and cell shape but not to growth properties.

摘要

使用抗成纤维细胞肌球蛋白抗体和间接免疫荧光显微镜检查细胞中微丝束的分布。微丝束的存在与正常生长控制之间没有相关性。在粘附性差的基质上培养的正常细胞系（Balb/c 3T3）未显示微丝束。同样，具有正常生长但由于对基质的粘附性缺陷而呈圆形的突变细胞系（AD6）也未显示束状形成。另一方面，两种具有扁平形态的转化细胞系（瑞士SV3T3和Balb MSV-85）显示出广泛的束状形成。当用增加对基质粘附性的CSP（正常细胞的主要表面糖蛋白）处理粘附性差的转化细胞系（MC5-5）时，细胞形成广泛的微丝束且生长没有任何下降。我们得出结论，微丝束的分布与对基质的粘附性和细胞形状有关，而与生长特性无关。