Blumberg W E, Eisinger J, Lamola A A, Zuckerman D M
J Lab Clin Med. 1977 Apr;89(4):712-23.
An inexpensive portable fluorometer has been designed which can be used to determine the concentration of zinc protoporphyrin (ZPP) in a drop of unprocessed blood deposited on a disposable glass slide. No volume measurement of the blood sample need be made. The instrument displays the ZPP level in micrograms per deciliter of blood accurately and reproducibly, automatically calibrating each determination by comparing the blood fluorescence with that of a stable dye. The instrument, which we call a hematofluorometer, is ideally suited for use in primary screening for childhood and occupational lead intoxication. It can be used by public health personnel after a few minutes of instruction. Comparisons of ZPP levels obtained with the use of the hematofluorometer with erythrocyte protoporphyrin levels obtained using accepted extraction methods are presented.
已设计出一种价格低廉的便携式荧光计，可用于测定一滴未经处理的血液中锌原卟啉（ZPP）的浓度，该血液滴在一次性载玻片上。无需对血样进行体积测量。该仪器能准确且可重复地显示每分升血液中ZPP的水平（以微克计），通过将血液荧光与一种稳定染料的荧光进行比较，自动校准每次测定。我们将该仪器称为血液荧光计，非常适合用于儿童和职业性铅中毒的初步筛查。经过几分钟的培训，公共卫生人员即可使用。本文还给出了使用血液荧光计获得的ZPP水平与采用公认提取方法获得的红细胞原卟啉水平的比较结果。
