利用电子显微镜对多头绒泡菌核DNA中的回文序列进行表征。
Characterization of foldback sequences in Physarum polycephalum nuclear DNA using the electron microscope.
作者信息
Hardman N, Jack P L
出版信息
Eur J Biochem. 1977 Apr 1;74(2):275-83. doi: 10.1111/j.1432-1033.1977.tb11391.x.
Abstract
An examination of the foldback fraction of nuclear DNA from Physarum polycephalum has been carried out using the electron microscope. Results show that the inverted repeat sequences responsible for the formation of foldback DNA range from 150-3000 bases in length, with a number-average size of 340 bases. About one-half of the inverted sequences form looped structures with loop sizes averaging 1200 bases in length. The distance between adjacent foldback sequences is estimated to be in the range 100-1500 bases.
摘要
利用电子显微镜对多头绒泡菌核DNA的回折部分进行了检测。结果表明，负责形成回折DNA的反向重复序列长度在150至3000个碱基之间，数均大小为340个碱基。约一半的反向序列形成环状结构，环的大小平均为1200个碱基。相邻回折序列之间的距离估计在100至1500个碱基范围内。
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