绵枣儿、黑麦和小鼠细胞核染色体中折返DNA序列的定位

Localisation of foldback DNA sequences in nuclei chromosomes of Scilla, Secale, and of mouse.

作者信息

Deumling B

出版信息

Nucleic Acids Res. 1978 Oct;5(10):3589-602. doi: 10.1093/nar/5.10.3589.

DOI:10.1093/nar/5.10.3589

PMID:569290

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342697/

Abstract

Foldback DNA, prepared from mouse and Scilla sibirica main band DNA, and from rye (Secale cereale) total DNA, was characterised by denaturation, renaturation, and electron microscopy. 3H-cRNA of this DNA was hybridised in situ to nuclei and chromosomes of the respective species. There is no universal labelling pattern among the three species. In mouse, highly repetitive foldback DNA is present in the whole chromatin including the satellite DNA-containing regions. In Scilla sibirica, on the contrary, the highly repetitive foldback sequences are excluded form the satellite DNA loci and are arranged in clusters in the remaining chromatin. In rye, there is a clear preferential labelling of the chromocenters in the interphase nuclei as well as metaphase chromosomes, indicating that highly repetitive foldback DNA is preferentially located among other highly repetitive sequences.

摘要

从小鼠和西伯利亚绵枣儿主带DNA以及黑麦（黑麦草）总DNA制备的回文DNA，通过变性、复性和电子显微镜进行了表征。该DNA的3H-cRNA原位杂交到相应物种的细胞核和染色体上。这三个物种之间没有普遍的标记模式。在小鼠中，高度重复的回文DNA存在于整个染色质中，包括含卫星DNA的区域。相反，在西伯利亚绵枣儿中，高度重复的回文序列被排除在卫星DNA位点之外，并在其余染色质中聚集成簇。在黑麦中，间期细胞核以及中期染色体的染色中心有明显的优先标记，表明高度重复的回文DNA优先位于其他高度重复序列之中。