Friedman R M
Science. 1967 Jun 30;156(3783):1760-1. doi: 10.1126/science.156.3783.1760.
Chick cells incubated at 1 degrees C with interferon fail to develop antiviral activity, but this activity appears subsequent to a 7-hour incubation at 37 degrees C after removal of interferon by repeated washings. Treatment with actinomycin D blocks the development of the latter activity. Cells incubated with interferon at 1 degrees C for up to 1 hour and then washed and incubated for 2 hours at 37 degrees C develop a degree of antiviral activity proportional to the concentration of interferon at initial incubation; at any concentration, the antiviral activity increased with the duration of initial incubation at 1 degrees C, but a maximal response was reached at 10 or 20 minutes. Treatment with trypsin after incubation with interferon at 1 degrees C inhibited development of antiviral activity. Interferon is rapidly bound to a superficial cell site, and this binding is necessary for development of antiviral activity in chick cells.
在1摄氏度下与干扰素一起孵育的鸡细胞无法产生抗病毒活性，但在通过反复洗涤去除干扰素后，在37摄氏度下孵育7小时后会出现这种活性。用放线菌素D处理会阻断后一种活性的产生。在1摄氏度下与干扰素一起孵育长达1小时，然后洗涤并在37摄氏度下孵育2小时的细胞，会产生与初始孵育时干扰素浓度成比例的抗病毒活性；在任何浓度下，抗病毒活性都随着在1摄氏度下初始孵育时间的延长而增加，但在10或20分钟时达到最大反应。在1摄氏度下与干扰素孵育后用胰蛋白酶处理会抑制抗病毒活性的产生。干扰素会迅速结合到细胞表面位点，这种结合对于鸡细胞中抗病毒活性的产生是必需的。
