Pang S F, Brown G M, Grota L J, Chambers J W, Rodman R L
Neuroendocrinology. 1977;23(1):1-13. doi: 10.1159/000122649.
An N-acetylserotonin/melatonin radioimmunoassay (NAS/Mel RIA) and a Mel RIA were developed to measure NAS and Mel contents in tissues of rats and chickens. Anti-NAS and anti-Mel sera were produced by immunization of rabbits with NAS-M-bovine serum albumin (BSA) and Mel-M-BSA, respectively. Anti-NAS serum used in the NAS/Mel RIA reacts equally well with NAS and Mel, while anti-Mel serum used in the Mel/RIA reacts specifically with Mel. The NAS and Mel levels in the pineal and Harderian gland, the retina and the brain of rats and chickens and Mel levels in chicken serum were determined using these RIAs. Levels of NAS and Mel in the rat pineal demonstrated diurnal rhythms with high levels during the dark period and low levels during the light period. The indole levels determined in this study correlate well with those obtained by other methods.
开发了一种N-乙酰血清素/褪黑素放射免疫测定法（NAS/Mel RIA）和一种Mel RIA，用于测量大鼠和鸡组织中的NAS和Mel含量。分别用NAS-M-牛血清白蛋白（BSA）和Mel-M-BSA免疫兔子，制备抗NAS和抗Mel血清。NAS/Mel RIA中使用的抗NAS血清与NAS和Mel反应同样良好，而Mel/RIA中使用的抗Mel血清仅与Mel特异性反应。使用这些放射免疫测定法测定了大鼠和鸡的松果体、哈德氏腺、视网膜和大脑中的NAS和Mel水平以及鸡血清中的Mel水平。大鼠松果体中的NAS和Mel水平呈现昼夜节律，黑暗期水平高，光照期水平低。本研究中测定的吲哚水平与其他方法获得的结果高度相关。
