Moss T, Stephens R M, Crane-Robinson C, Bradbury E M
Nucleic Acids Res. 1977 Jul;4(7):2477-85. doi: 10.1093/nar/4.7.2477.
The low-angle X-ray diffraction pattern from fibres of reconstituted H3/H4/DNA complexes is very similar to that of chromatin and has well defined maxima at 10.6, 5.4, 3.4 and 2.6 nm. Staphyloccal nuclease digestion of reconstituted H3/H4/DNA yields DNA fragments of length 49, 69, 100, 128, 193 and 255 b.p. as principal components. Comparison of the relative amounts of DNA fragments shows that the larger components (100 and 128 b.p.) increase with respect to the smaller (49 and 69 b.p.) as the histone to DNA ratio increases. A structural unit containing intergral of 65 b.p. of DNA and tetrameric (H3/H4)2 is proposed such that longer DNA fragments result from multiples of this unit. The principal nucleo-protein particle resulting from nuclease digestion contains 128/139 b.p. of DNA and has electrophoretic mobility very close to that of 'core' nucleosome. It probably represents a dimer of the basic structural unit.
重构的H3/H4/DNA复合物纤维的低角度X射线衍射图谱与染色质的图谱非常相似，在10.6、5.4、3.4和2.6纳米处有明确的最大值。用葡萄球菌核酸酶消化重构的H3/H4/DNA，产生长度为49、69、100、128、193和255碱基对的DNA片段作为主要成分。DNA片段相对量的比较表明，随着组蛋白与DNA比例的增加，较大的成分（100和128碱基对）相对于较小的成分（49和69碱基对）会增加。有人提出一种结构单元，它包含65碱基对的DNA和四聚体（H3/H4）2，这样较长的DNA片段就是这个单元的倍数。核酸酶消化产生的主要核蛋白颗粒含有128/139碱基对的DNA，其电泳迁移率与“核心”核小体非常接近。它可能代表基本结构单元的二聚体。
