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在鸡红细胞染色质有限的DNA酶1消化过程中染色体蛋白的选择性释放。

Selective release of chromosomal proteins during limited DNAase 1 digestion of avian erythrocyte chromatin.

作者信息

Vidali G, Boffa L C, Allfrey V G

出版信息

Cell. 1977 Oct;12(2):409-15. doi: 10.1016/0092-8674(77)90117-9.

DOI:10.1016/0092-8674(77)90117-9
PMID:562236
Abstract

Duck erythrocyte chromatin has been treated with DNAase 1 under conditions that are known to digest selectively the structural genes coding for globin mRNAs. This limited digestion releases specific sets of nonhistone chromosomal proteins that are not preferentially released during limited digestion with micrococcal nuclease, which does not selectively attack the globin sequences. Analysis of nucleosome monomer and multimer peaks separated on sucrose gradients after limited digestion with micrococcal nuclease shows that the proteins which are released by DNAase 1 digestion remain associated with the chromatin subunits and can be removed by extraction in 0.5 M NaCl. These proteins are tentatively identified as members of the high mobility group (HMG) proteins (originally described by Goodwin, Sanders and Johns, 1973) in terms of their extractability, electrophoretic characteristics and amino acid composition.

摘要

鸭红细胞染色质已在已知能选择性消化编码珠蛋白mRNA的结构基因的条件下用DNA酶1处理。这种有限的消化释放出特定的非组蛋白染色体蛋白组，在用微球菌核酸酶进行有限消化时不会优先释放这些蛋白，微球菌核酸酶不会选择性攻击珠蛋白序列。对微球菌核酸酶有限消化后在蔗糖梯度上分离的核小体单体和多聚体峰的分析表明，DNA酶1消化释放的蛋白质仍与染色质亚基结合，并且可以通过在0.5M NaCl中提取而去除。根据它们的可提取性、电泳特性和氨基酸组成，这些蛋白质初步被鉴定为高迁移率族（HMG）蛋白成员（最初由古德温、桑德斯和约翰斯于1973年描述）。

相似文献

1
Selective release of chromosomal proteins during limited DNAase 1 digestion of avian erythrocyte chromatin.在鸡红细胞染色质有限的DNA酶1消化过程中染色体蛋白的选择性释放。
Cell. 1977 Oct;12(2):409-15. doi: 10.1016/0092-8674(77)90117-9.
2
Fractionation by micrococcal nuclease digestion of Drosophila embryo chromatin: isolation of a fraction enriched in two major nonhistone proteins.通过微球菌核酸酶消化果蝇胚胎染色质进行分级分离：分离出富含两种主要非组蛋白的组分。
Cell Differ. 1983 Jun;12(6):307-16. doi: 10.1016/0045-6039(83)90009-x.
3
Selective release of HMG nonhistone proteins during DNase digestion of Tetrahymena chromatin at different stages of the cell cycle.在细胞周期不同阶段，四膜虫染色质经脱氧核糖核酸酶消化时HMG非组蛋白的选择性释放。
Nucleic Acids Res. 1980 Nov 25;8(22):5275-88. doi: 10.1093/nar/8.22.5275.
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A study of the localization of high mobility group proteins in chromatin.一项关于高迁移率族蛋白在染色质中定位的研究。
Can J Biochem. 1978 Jun;56(6):480-91. doi: 10.1139/o78-075.
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Isolation of a subclass of nuclear proteins responsible for conferring a DNase I-sensitive structure on globin chromatin.分离出一类负责赋予珠蛋白染色质对核酸酶I敏感结构的核蛋白亚类。
Proc Natl Acad Sci U S A. 1979 Feb;76(2):630-4. doi: 10.1073/pnas.76.2.630.
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Fractionation of nucleosomes by salt elution from micrococcal nuclease-digested nuclei.通过从微球菌核酸酶消化的细胞核中进行盐洗脱来分离核小体。
J Cell Biol. 1978 Oct;79(1):97-109. doi: 10.1083/jcb.79.1.97.
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A protein released by DNAase I digestion of drosophila nuclei is preferentially associated with puffs.经脱氧核糖核酸酶I消化果蝇细胞核后释放出的一种蛋白质，优先与疏松染色区相关联。
Cell. 1978 Jul;14(3):539-44. doi: 10.1016/0092-8674(78)90240-4.
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Are the high mobility group non-histone chromosomal proteins associated with 'active' chromatin?高迁移率族非组蛋白染色体蛋白与“活性”染色质有关吗？
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Effects of thyrotropin on the phosphorylation of histones and nonhistone phosphoproteins in micrococcal nuclease-sensitive and resistant thyroid chromatin.促甲状腺激素对微球菌核酸酶敏感和抗性甲状腺染色质中组蛋白和非组蛋白磷酸化蛋白磷酸化的影响。
Endocrinology. 1983 May;112(5):1816-22. doi: 10.1210/endo-112-5-1816.
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Selective association of the trout-specific H6 protein with chromatin regions susceptible to DNase I and DNase II: possible location of HMG-T in the spacer region between core nucleosomes.鳟鱼特异性H6蛋白与对DNase I和DNase II敏感的染色质区域的选择性关联：HMG-T在核心核小体之间间隔区域的可能定位。
Proc Natl Acad Sci U S A. 1977 Jul;74(7):2810-4. doi: 10.1073/pnas.74.7.2810.

引用本文的文献

1
Blotting Index of Dissimilarity: use to study immunological relatedness of plant and animal High Mobility Group (HMG) chromosomal proteins.不相似度印迹指数：用于研究植物和动物高迁移率族（HMG）染色体蛋白的免疫相关性。
Plant Mol Biol. 1987 Sep;9(5):431-42. doi: 10.1007/BF00015875.
2
Heterogeneous binding of high mobility group chromosomal proteins to nuclei.高迁移率族染色体蛋白与细胞核的异质性结合。
J Cell Biol. 1981 Feb;88(2):373-9. doi: 10.1083/jcb.88.2.373.
3
Immuno-biochemical studies of a non-histone chromosomal protein in embryonic and mature chick oviduct.
胚胎期和成熟鸡输卵管中一种非组蛋白染色体蛋白的免疫生化研究。
Biochem J. 1980 Jan 1;185(1):169-75. doi: 10.1042/bj1850169.
4
Immunochemical study of chromatin non-histone proteins. II. Localization of immunogenic tissue-specific proteins in nuclease-hypersensitive sites of chromatin.染色质非组蛋白的免疫化学研究。II. 免疫原性组织特异性蛋白在染色质核酸酶超敏位点中的定位。
Mol Cell Biochem. 1984 Jun;62(2):141-7. doi: 10.1007/BF00223304.
5
Changes in quantities of high-mobility-group protein 1 in oviduct cellular fractions after oestrogen stimulation.雌激素刺激后输卵管细胞组分中高迁移率族蛋白1含量的变化
Biochem J. 1981 Jul 15;198(1):85-90. doi: 10.1042/bj1980085.
6
Differential phosphorylation of nuclear nonhistone high mobility group proteins HMG 14 and HMG 17 during the cell cycle.细胞周期中核内非组蛋白高迁移率族蛋白HMG 14和HMG 17的差异磷酸化作用
Proc Natl Acad Sci U S A. 1981 Nov;78(11):6944-8. doi: 10.1073/pnas.78.11.6944.
7
Loss of chromosomal high mobility group proteins HMG1 and HMG2 when mouse neuroblastoma and Friend erythroleukemia cells become committed to differentiation.当小鼠神经母细胞瘤细胞和弗瑞德红白血病细胞开始分化时，染色体高迁移率族蛋白HMG1和HMG2的缺失。
Proc Natl Acad Sci U S A. 1981 Oct;78(10):5988-92. doi: 10.1073/pnas.78.10.5988.
8
Comparative studies on microinjected high-mobility-group chromosomal proteins, HMG1 and HMG2.微注射的高迁移率族染色体蛋白HMG1和HMG2的比较研究
J Cell Biol. 1981 Nov;91(2 Pt 1):488-96. doi: 10.1083/jcb.91.2.488.
9
Partial inhibition of histone deacetylase in active chromatin by HMG 14 and HMG 17.HMG 14和HMG 17对活性染色质中组蛋白脱乙酰酶的部分抑制作用。
Nucleic Acids Res. 1980 May 10;8(9):1947-63. doi: 10.1093/nar/8.9.1947.
10
High mobility group proteins 1 and 2 are present in simian virus 40 provirions, but not in virions.高迁移率族蛋白1和2存在于猿猴病毒40前病毒颗粒中，但不存在于病毒颗粒中。
Nucleic Acids Res. 1981 Jan 10;9(1):121-31. doi: 10.1093/nar/9.1.121.