Cook B, Hunter R H, Kelly A S
J Reprod Fertil. 1977 Sep;51(1):65-71. doi: 10.1530/jrf.0.0510065.
No unusual steroid-binding proteins that might react with the oocyte or its investments could be detected in follicular fluid. Corticosteroid-binding globulin occurred in follicular fluid from pigs, sheep and cows, and sex hormone-binding globulin occurred in follicular fluid from sheep and cows. The bulk of the steroid in follicular fluid is bound to albumin with low affinity, indicating that steroid molecules can readily be released, and oestrogen can react with the oocyte and granulosa cells in a manner analogous to that demonstrated for target cells bathed with interstitial fluid. Pigs lack a sex-hormone binding globulin in blood plasma and, hence, in follicular fluid. Because no proteins exist in follicular fluid that would compete with antibodies to bind steroids, direct radioimmunoassay of follicular steroids appears to be a valid technique.
在卵泡液中未检测到可能与卵母细胞或其附属结构发生反应的异常甾体结合蛋白。皮质类固醇结合球蛋白存在于猪、绵羊和奶牛的卵泡液中，而性激素结合球蛋白存在于绵羊和奶牛的卵泡液中。卵泡液中的大部分甾体以低亲和力与白蛋白结合，这表明甾体分子可以很容易地释放出来，并且雌激素能够以类似于用间质液灌注的靶细胞所显示的方式与卵母细胞和颗粒细胞发生反应。猪的血浆中缺乏性激素结合球蛋白，因此其卵泡液中也没有。由于卵泡液中不存在能与抗体竞争结合甾体的蛋白质，因此卵泡甾体的直接放射免疫测定似乎是一种有效的技术。
