Byers V S, Sercarz E E
J Exp Med. 1968 Feb 1;127(2):307-25. doi: 10.1084/jem.127.2.307.
A set of conditions has been described under which primed rabbit lymph nodes produce a secondary antibody response upon in vivo stimulation with a large dose of antigen, but are subsequently "exhausted;" that is, lymph node cultures prepared at intervals following the booster injection cannot be re-stimulated to display tertiary responses. Rabbits given 100-fold less antigen in the booster inoculum were able to give a tertiary response upon in vitro challenge. The system used permits neither induction nor continuation of a primary response to BSA in vitro. Since it could be demonstrated that no memory cells were generated by the booster injection within the intervals between in vivo injection and culture, the tertiary response in nonexhausted nodes must have been due to residual memory cells which remained untriggered by the in vivo booster injection. The unresponsive state was not caused by antibody feedback. These results are interpreted to mean that a population of memory cells can be exhausted by a supraoptimal dose of antigen, rendering the node temporarily incapable of further response. This implies that long-lived memory is not due to asymmetric division of memory cells. The source and fate of memory cells is discussed with regard to this evidence.
已经描述了一组条件，在这些条件下，致敏的兔淋巴结在体内用大剂量抗原刺激后会产生二次抗体反应，但随后会“耗尽”；也就是说，在加强注射后的不同时间间隔制备的淋巴结培养物不能被再次刺激以显示三次反应。在加强接种物中给予抗原量少100倍的兔子能够在体外攻击时产生三次反应。所使用的系统既不能在体外诱导对牛血清白蛋白的初次反应，也不能使其持续。由于可以证明在体内注射和培养之间的间隔内，加强注射不会产生记忆细胞，因此未耗尽的淋巴结中的三次反应一定是由于残留的记忆细胞，这些记忆细胞未被体内加强注射触发。无反应状态不是由抗体反馈引起的。这些结果被解释为意味着一群记忆细胞可以被超最佳剂量的抗原耗尽，使淋巴结暂时无法进一步反应。这意味着长期记忆不是由于记忆细胞的不对称分裂。关于这一证据，讨论了记忆细胞的来源和命运。
