Worthington B S, Meserole L, Syrotuck J A
Am J Dig Dis. 1978 Jan;23(1):23-32. doi: 10.1007/BF01072571.
The effects of regular ethyl alcohol ingestion on morphological and permeability characteristics of the small intestine were assessed in mature rats using the tracer protein, horseradish peroxidase. Thirty adult rats were divided into two groups and provided a standard commercial diet in pellet form. Each morning, after an overnight fast, every animal in the experimental group was administered by gavage an aliquot of 20% ethanol; animals in the control group were provided aliquots of 20% sucrose in water by the same method. After 4 and 8 weeks on the gavage routine (and 10 days and 4 weeks after gavage cessation), jejunal permeability to horseradish peroxidase was examined in animals from each group. Using a routine ligated-loop procedure and light and electron microscopy, ethanol-exposed rats demonstrated increased intestinal permeability to horseradish peroxidase by 4 weeks; sucrose-exposed animals revealed little alteration in mucosal integrity. It is proposed that regular ingestion of sizable amounts of alcohol alters morphological characteristics of the gut and increase the permeability of the mucosa to "undigested" macromolecules.
在成年大鼠中，使用示踪蛋白辣根过氧化物酶评估了定期摄入乙醇对小肠形态和通透性特征的影响。30只成年大鼠被分为两组，并提供颗粒状的标准商业饮食。每天早晨，在禁食过夜后，实验组的每只动物通过灌胃给予一份20%乙醇；对照组的动物通过相同方法给予等量的20%蔗糖水溶液。在进行4周和8周的灌胃程序后（以及停止灌胃10天和4周后），检测每组动物空肠对辣根过氧化物酶的通透性。采用常规的结扎肠袢程序以及光镜和电镜观察，发现乙醇处理的大鼠在4周时小肠对辣根过氧化物酶的通透性增加；蔗糖处理的动物黏膜完整性几乎没有改变。有人提出，定期摄入大量酒精会改变肠道的形态特征，并增加黏膜对“未消化”大分子的通透性。
