Chorbanian A V
J Clin Invest. 1968 Mar;47(3):595-603. doi: 10.1172/JCI105755.
Intimal sterol synthesis was examined in isolated human arterial segments obtained at surgery or at postmortem examination. The tissues were incubated with acetate-1-(14)C and mevalonate-2-(14)C and the incorporation of these precursors into sterols was determined. Intimal sterols were isolated by multiple chromatographic techniques and purified by bromination and oxidation procedures. The results indicate that the arterial intima can incorporate acetate and mevalonate into cholesterol, cholestanol, and squalene. Cholestanol was the major sterol synthesized by the arterial wall, but cholesterol production was also consistently observed. The findings suggest that local synthesis is a potential source of sterol accumulation within the arterial wall. The conversion of cholesterol to other sterols was also studied in terminally ill patients receiving labeled cholesterol before death. Tissue analyses revealed the presence of labeled cholestanol as well as cholesterol in the tissue 5-104 days after labeled cholesterol administration. The results demonstrate the conversion of cholesterol to cholestanol in man and suggest that the exchange of cholestanol between the blood and tissues is similar to that of cholesterol.
在手术或尸检时获取的离体人体动脉节段中检测内膜固醇的合成。将组织与乙酸盐 -1-(14)C 和甲羟戊酸 -2-(14)C 一起孵育，并测定这些前体掺入固醇中的情况。通过多种色谱技术分离内膜固醇，并通过溴化和氧化程序进行纯化。结果表明，动脉内膜可将乙酸盐和甲羟戊酸掺入胆固醇、胆甾烷醇和角鲨烯中。胆甾烷醇是动脉壁合成的主要固醇，但也始终观察到胆固醇的生成。这些发现表明，局部合成是动脉壁内固醇积累的一个潜在来源。还对临终前接受标记胆固醇的患者进行了胆固醇向其他固醇转化的研究。组织分析显示，在给予标记胆固醇后 5 - 104 天，组织中存在标记的胆甾烷醇以及胆固醇。结果证明了人体中胆固醇向胆甾烷醇的转化，并表明血液与组织之间胆甾烷醇的交换与胆固醇的交换相似。
