Shadduck J A
In Vitro. 1977;13(12):849-56. doi: 10.1007/BF02615134.
Experimental tumors developed in white Pekin ducks after intramuscular implantation of 20-methylcholanthrene. Cells derived from the primary tumor were adapted successfully to grow in vitro and have growth characteristics similar to that of established cell lines of mammalian origin. The cell density rises rapidly and the doubling time is approximately 17 hr. The duck cells have been cultured successfully for at least 80 passages in votro. The continuously cultured cells have the characteristic chromosome pattern of duck, and the DNA of the duck cell line hybridized with duck liver DNA. We believe we have established a continuous cell line of avian origin. Electron-microscopic examinations of the tumor cells and RNA-directed DNA polymerase of the cell-free supernate show no evidence of endogenous virus production.
在白色北京鸭肌肉注射20-甲基胆蒽后产生了实验性肿瘤。从原发性肿瘤中分离出的细胞成功适应了体外生长，并且具有与已建立的哺乳动物来源细胞系相似的生长特性。细胞密度迅速上升，倍增时间约为17小时。鸭细胞已在体外成功培养了至少80代。连续培养的细胞具有鸭的特征性染色体模式，并且鸭细胞系的DNA与鸭肝DNA杂交。我们相信我们已经建立了一个禽类来源的连续细胞系。对肿瘤细胞的电子显微镜检查以及无细胞上清液中的RNA指导的DNA聚合酶均未显示内源性病毒产生的证据。
