宿主诱导的禽肉瘤病毒B-77基因组改变。
Host induced alteration of avian sarcoma virus B-77 genome.
作者信息
Shoyab M, Markham P D, Baluda M A
出版信息
Proc Natl Acad Sci U S A. 1975 Mar;72(3):1031-5. doi: 10.1073/pnas.72.3.1031.
The genome of an avian oncornavirus was altered after infection of a heterologous host. This was studied with avian sarcoma virus B-77 in duck embryonic fibroblasts (DEF) and chicken embryonic fibroblasts (CEF). To detect alteration of the viral genome, we hybridized 35S B-77 RNA with normal duck DNA by either one of two techniques:when viral RNA was in excess and when DNA was in excess. The RNA of B-77 passaged only in gs minus chf minus CEF does not have homology with duck DNA. However, after several passages of B-77 through DEF the viral genome acquired duck specific RNA sequences. After 4 and 10 passages, B-77 RNA acquired 2.2 and 6.6%, respectively, complementarity to normal duck DNA. The duck specific RNA sequences were found to be covalently linked to the B-77 RNA genome. Also, the host specific sequences acquired by the virus appear to be from a region of the duck DNA which is repeated four to six times per cell. After 5 back passages in CEF some of the duck specific RNA sequences in the viral genome were lost.
一种禽肿瘤病毒在感染异源宿主后，其基因组发生了改变。利用禽肉瘤病毒B - 77在鸭胚成纤维细胞（DEF）和鸡胚成纤维细胞（CEF）中对此进行了研究。为了检测病毒基因组的改变，我们通过两种技术之一，即当病毒RNA过量时和当DNA过量时，使35S B - 77 RNA与正常鸭DNA杂交。仅在gs阴性chf阴性CEF中传代的B - 77的RNA与鸭DNA没有同源性。然而，B - 77在DEF中传代几次后，病毒基因组获得了鸭特异性RNA序列。传代4次和10次后，B - 77 RNA分别获得了与正常鸭DNA 2.2%和6.6%的互补性。发现鸭特异性RNA序列与B - 77 RNA基因组共价连接。此外，病毒获得的宿主特异性序列似乎来自鸭DNA的一个区域，该区域在每个细胞中重复4至6次。在CEF中回传5次后，病毒基因组中的一些鸭特异性RNA序列丢失了。
Zotero 插件