Reed C F
J Clin Invest. 1968 Apr;47(4):749-60. doi: 10.1172/JCI105770.
The turnover of the four major erythrocyte phospholipids has been studied with (32)P, both in vivo and in vitro, in man and the dog. Phosphatidyl serine and phosphatidyl ethanolamine appeared to be stable erythrocyte lipids in both species. Turnover of the phosphate moiety of lecithin and sphingomyelin in the circulating erythrocytes of these two species seems entirely due to an exchange of the whole molecule with the corresponding plasma compound. Exchangeable and nonexchangeable pools of these two cellular lipids were found. In man about 60% of erythrocyte lecithin is exchangeable. The 12 hr fractional turnover of this pool is approximately 13%. Only 30% of the sphingomyelin in human cells appeared exchangeable; this portion had a 12 hr fractional turnover of about 14%. Similar results were obtained in the dog except that in this species about 75% of the erythrocyte sphingomyelin was exchangeable. Inorganic (32)P was not incorporated into any of the four major phospholipids in either species. The present findings aid in estimating quantitatively the effect of plasmaerythrocyte lipid exchange on red blood cell phospholipids.
利用³²P对人和狗体内外四种主要红细胞磷脂的周转率进行了研究。磷脂酰丝氨酸和磷脂酰乙醇胺在这两个物种中似乎都是稳定的红细胞脂质。这两个物种循环红细胞中卵磷脂和鞘磷脂的磷酸部分的周转率似乎完全是由于整个分子与相应血浆化合物的交换。发现了这两种细胞脂质的可交换池和不可交换池。在人类中，约60%的红细胞卵磷脂是可交换的。该池的12小时分数周转率约为13%。人类细胞中只有30%的鞘磷脂似乎是可交换的；这部分的12小时分数周转率约为14%。在狗身上也得到了类似的结果，只是在这个物种中，约75%的红细胞鞘磷脂是可交换的。无机³²P在这两个物种中都没有掺入任何一种主要磷脂中。目前的研究结果有助于定量估计血浆-红细胞脂质交换对红细胞磷脂的影响。
