Steck T L, Wackman N, Tarlov A R
J Supramol Struct. 1976;4(2):169-80. doi: 10.1002/jss.400040204.
Intact rat or human erythrocytes and their isolated (ghost) membranes were incubated with the high speed supernatant fraction of homogenates derived from 32P-labeled rat livers. Phospholipid molecules were transferred between the red cell membranes and the liver extracts, as reflected by the convergence of their specific radioactivities with time. Whereas ghosts usually approached isotopic equilibrium with the liver supernatant fraction during a few hours of incubation at 37 degrees C, the exchange of phospholipids by intact cells was no more than one-half, even after 18 hr. Phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine and sphingomyelin were all exchanged in both intact cells and ghosts, albeit to different extents. (A control experiment, incubating 32P-labeled rat erythrocytes or ghosts with unlabeled rat liver extracts, also demonstrated the exchange of all four major phospholipids.) These data may signify that the phospholipids on the cytoplasmic side of the membrane of intact erythrocytes do not exchange with the phospholipids in exogenous liver extracts. If so, all four major phospholipid classes would appear to be present to some extent at both membrane surfaces. The first inference is in agreement with several other studies on this membrane, while the second inference is not.
将完整的大鼠或人类红细胞及其分离的（血影）膜与来自32P标记大鼠肝脏匀浆的高速上清液部分一起孵育。磷脂分子在红细胞膜和肝脏提取物之间转移，这可通过它们的比放射性随时间的趋同来反映。在37℃孵育数小时期间，血影通常与肝脏上清液部分达到同位素平衡，而完整细胞的磷脂交换即使在18小时后也不超过一半。磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰丝氨酸和鞘磷脂在完整细胞和血影中均有交换，尽管程度不同。（一项对照实验，将32P标记的大鼠红细胞或血影与未标记的大鼠肝脏提取物一起孵育，也证明了所有四种主要磷脂的交换。）这些数据可能表明完整红细胞膜细胞质侧的磷脂不会与外源性肝脏提取物中的磷脂交换。如果是这样，所有四种主要磷脂类似乎在膜的两个表面都有一定程度的存在。第一个推断与关于该膜的其他几项研究一致，而第二个推断则不然。
