Kimberg D V, Loud A V, Wiener J
J Cell Biol. 1968 Apr;37(1):63-79. doi: 10.1083/jcb.37.1.63.
The effects of cortisone treatment on oxygen consumption, oxidative phosphorylation, and fine structure of rat liver mitochondria have been studied. Male rats weighing 125 g were treated for 6 days with 5 mg of cortisone acetate or isotonic saline. On the 7th day, sections of liver were excised and processed for light and electron microscopy. Mitochondrial respiration and oxidative phosphorylation were studied with mitochondria isolated from these livers. Cortisone treatment is responsible for a 14-40% decrease in the amount of oxygen consumed per mg of mitochondrial protein when succinate, alpha-ketoglutarate, or beta-hydroxybutyrate are used as substrates, or with ascorbate and N,N,N(1),N(1)-tetramethyl p-phenylenediamine as electron donors. In addition, oxidative phosphorylation is uncoupled with a lowering of the P:O ratios. Randomly selected liver cells have been analyzed by quantitative morphometric techniques. The average mitochondrial volume is increased fourfold in the peripheral and midzonal regions with a commensurate decrease in the number of mitochondria per cell. These alterations are present throughout the hepatic lobule, but are most marked in midzonal cells. The total mitochondrial volume per cell and the per cent of the total cytoplasmic volume occupied by mitochondria remains relatively unaltered, as does the total amount of cristae surface per cell. While the mitochondria are enlarged, they are not "swollen." The relationships between the steroid hormone treatment and the alterations in mitochondrial function and structure are discussed.
已经研究了可的松治疗对大鼠肝脏线粒体的氧消耗、氧化磷酸化和精细结构的影响。对体重125克的雄性大鼠用5毫克醋酸可的松或等渗盐水治疗6天。在第7天，切除肝脏切片并进行光镜和电镜处理。用从这些肝脏中分离出的线粒体研究线粒体呼吸和氧化磷酸化。当使用琥珀酸、α-酮戊二酸或β-羟基丁酸作为底物，或以抗坏血酸和N,N,N(1),N(1)-四甲基对苯二胺作为电子供体时，可的松治疗导致每毫克线粒体蛋白的氧消耗量减少14%-40%。此外，氧化磷酸化解偶联，P:O比值降低。已通过定量形态计量技术对随机选择的肝细胞进行了分析。外周和中区区域的线粒体平均体积增加了四倍，每个细胞中的线粒体数量相应减少。这些改变存在于整个肝小叶中，但在中区细胞中最为明显。每个细胞的线粒体总体积以及线粒体占细胞质总体积的百分比保持相对不变，每个细胞的嵴表面总量也保持不变。虽然线粒体增大了，但它们并没有“肿胀”。讨论了类固醇激素治疗与线粒体功能和结构改变之间的关系。
