Wilkins P O
J Bacteriol. 1967 May;93(5):1565-70. doi: 10.1128/jb.93.5.1565-1570.1967.
The initial rate of the glucose-induced countertransport of d-arabinose was measured at several concentrations of extracellular glucose. These data permit the calculation of the intracellular concentration of free glucose, and, if the rate of glucose metabolism is known, the maximal rate of glucose transport can be estimated. Since the maximal transport rate remained essentially constant when the extracellular glucose concentration was increased from 2 to 100 mm, the results are consistent with the hypothesis that, during glucose metabolism, glucose is transported across the yeast cell membrane by a symmetrical carrier system which functions independently of metabolism.
在几种细胞外葡萄糖浓度下测量了葡萄糖诱导的D-阿拉伯糖反向转运的初始速率。这些数据有助于计算细胞内游离葡萄糖的浓度,并且,如果已知葡萄糖代谢速率,就可以估算葡萄糖转运的最大速率。由于当细胞外葡萄糖浓度从2 mM增加到100 mM时,最大转运速率基本保持恒定,因此这些结果与以下假设一致:在葡萄糖代谢过程中,葡萄糖通过一个对称载体系统跨酵母细胞膜转运,该系统独立于代谢发挥作用。